Mdoyle Week 9

Assignment Page

BIOL367/F10:GenMAPP and MAPPFinder Protocols

Electronic Lab Notebook

Top 10 GO Terms:

1. hexose catabolic process
2. glucose catabolic process
3. glycolysis
4. monosaccharide catabolic process
5. cytoplasm
6. alcohol catabolic process
7. cellular carbohydrate catabloic process
8. glucose metabolic process
9. protein folding
10. hexose metabolic process

Compare with a buddy: Salomon's terms were different from mine, because of the changes made to the newer version of the gene database that I was using.

VC0028

1. branched chain family amino acid biosynthetic process
2. cellular amino acid biosynthetic process
3. metabolic process
4. metal ion binding
5. iron-sulfur cluster binding
6. 4 iron, 4 sulfur cluster binding
7. catalytic activity
8. lyase activity
9. dihydroxy-acid dehydratase activity

VC0941

1. glycine metabolic process
2. L-serine metabolic process
3. one-carbon metabolic process
4. cytoplasm
5. pyridoxal phosphate binding
6. transferase activity
7. glycine hydroxymethyltransferase activity

VC0869

1. glutamine metabolic process
2. purine nucleotide biosynthetic process
3. 'de novo' IMP biosynthetic process
4. cytoplasm
5. nucleotide binding
6. ATP binding
7. catalytic activity
8. ligase activity
9. phosphoribosylformylglycinamidine synthase activity

VC0051

1. purine nucleotide biosynthetic process
2. 'de novo' IMP biosynthetic process
3. nucleotide binding
4. ATP binding
5. catalytic activity
6. lyase activity
7. carboxy-lyase activity
8. phosophoribosylaminoimidazole carboxylase activity

VC0647

1. mRNA catabolic process
2. RNA processing
3. cytoplasm
4. mitochondrion
5. RNA binding
6. 3'-5'-exoribonuclease activity
7. transferase activity
8. nucleotidyltransferase activity
9. polyribonucleotide nucleotidyltransferase activity

VC0468

1. glutathione biosynthetic process
2. metal ion binding
3. nucleotide binding
4. ATP binding
5. catalytic activity
6. ligase activity
7. glutathione synthase activity

VC2350

1. deoxyribonucleotide catabolic process
2. metabolic process
3. cytoplasm
4. catalytic activity
5. lyase activity
6. deoxyribose-phosphate aldolase activity

VCA0583

1. transport
2. outer membrane-bound periplasmic space
3. transporter activity

Compare with buddy: My partner got different terms again, I assume due to the fact that several changes have been made between the old and new versions of the gene database.

The GO term I chose was VCA0583, and the expression of the gene appeared to have increased fairly significantly. The function of the gene is that it is involved in the storage or transport of lipids necessary for membrane maintenance under stressful conditions. It also displays a binding preference for lysophospholipids