Media Recipes: Difference between revisions
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=='''1x NMS1 - 1L'''== | =='''1x NMS1 - 1L'''== | ||
''(correct as of | ''(correct as of 06/13/13)'' | ||
1. Dissolve in ddH2O: | 1. Dissolve in ddH2O: | ||
:*1.00 g MgSO4•7H2O | :*1.00 g MgSO4•7H2O | ||
:*0.2 g CaCl2•6H2O | :*0.2 g CaCl2•6H2O (''or'' 0.14 g CaCl2•2H2O) | ||
:*1.00 g KNO3 | :*1.00 g KNO3 | ||
2. Fill to 1000 mL | 2. Fill to 1000 mL | ||
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=='''1x NMS2 - 1L'''== | =='''1x NMS2 - 1L'''== | ||
''(correct as of | ''(correct as of 06/13/13)'' | ||
1. Dissolve in ddH2O: | 1. Dissolve in ddH2O: | ||
:*1.00 g MgSO4•7H2O | :*1.00 g MgSO4•7H2O | ||
:*0.02 g CaCl2•6H2O | :*0.02 g CaCl2•6H2O (''or'' 0.014 g CaCl2•2H2O) | ||
:*1.00 g KNO3 | :*1.00 g KNO3 | ||
:*7.50 g NaCl | :*7.50 g NaCl | ||
Line 28: | Line 28: | ||
=='''1x NMS Trace Elements - 1L'''== | =='''1x NMS Trace Elements - 1L'''== | ||
(correct as of | (correct as of 06/13/13) | ||
'''''Note that trace elements should be remade approximately every 3 months''''' | '''''Note that trace elements should be remade approximately every 3 months''''' |
Latest revision as of 14:31, 14 June 2013
1x NMS1 - 1L
(correct as of 06/13/13)
1. Dissolve in ddH2O:
- 1.00 g MgSO4•7H2O
- 0.2 g CaCl2•6H2O (or 0.14 g CaCl2•2H2O)
- 1.00 g KNO3
2. Fill to 1000 mL
3. Add 2 mL NMS Trace Elements
4. Autoclave
1x NMS2 - 1L
(correct as of 06/13/13)
1. Dissolve in ddH2O:
- 1.00 g MgSO4•7H2O
- 0.02 g CaCl2•6H2O (or 0.014 g CaCl2•2H2O)
- 1.00 g KNO3
- 7.50 g NaCl
2. Fill to 1000 mL 3. Add 2 mL NMS Trace Elements 4. Autoclave
1x NMS Trace Elements - 1L
(correct as of 06/13/13)
Note that trace elements should be remade approximately every 3 months
1. Dissolve in ddH2O:
- 1.0g Na2-EDTA
- 2.0g FeSO4•7H2O
- 0.8g ZnSO4•7H2O
- 0.03g MnCl2•4H2O
- 0.03g H3BO3
- 0.2g CoCl2•6H2O
- 0.6g CuCl2•2H2O
- 0.02g NiCl2•6H2O
- 0.05g Na2MoO•2H2O
2. Fill to 1L
3. Autoclave
4. Divide into 250 ml aliquots and label one as “In Use”
5. Store in refrigerator