Mesoplasma florum:Sequence Analysis

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(Sequencing results for transposon insertion events)
Current revision (18:58, 15 May 2008) (view source)
 
(43 intermediate revisions not shown.)
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 +
Spreadsheet with Data
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[[Image:MF Transposon Insertions.xls]]
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 +
[[Image:TI-287-383.xls]]
 +
 +
[[Image:TI-601-696.xls]]
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 +
Insertion locations sorted and genes knocked out identified: [[Image:MF-transposon-insertions.xls]]
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===Sequencing results for transposon insertion events===
===Sequencing results for transposon insertion events===
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!Gene's Locus Tag
!Gene's Locus Tag
!Gene ID
!Gene ID
-
!width="100"|'''Exact Insertion Site'''
+
!width="115"|'''Exact Insertion Site'''
 +
!Orientation of Transposon
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<sub>In relation to the direction of the genome, not of the gene</sub>
|-
|-
-
|T01I0001
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|[[T01I0001]]
-
|
+
|sucrose PTS system IIBC component
-
|
+
|Mfl516
-
|
+
|2897720
-
|
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|After base 608314
 +
|Backward
|-
|-
-
|T01I0002
+
|[[T01I0002]]
|beta-glucosidase
|beta-glucosidase
|Mfl011
|Mfl011
|2898258
|2898258
-
|
+
|After base 15546
 +
|Backward
|-
|-
-
|T01I0003
+
|[[T01I0003]]
|sucrose PTS system IIBC component
|sucrose PTS system IIBC component
|Mfl516
|Mfl516
|2897720
|2897720
-
|
+
|After base 608314
 +
|Backward
|-
|-
-
|T01I0004
+
|[[T01I0004]]
|unknown substrate ABC transporter permease component
|unknown substrate ABC transporter permease component
|Mfl159
|Mfl159
|2898241
|2898241
-
|
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|After base 179139
 +
|Forward
|-
|-
-
|T01I0005
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|[[T01I0005]]
|putative transcriptional regulator
|putative transcriptional regulator
|Mfl311
|Mfl311
|2897948
|2897948
-
|
+
|After base 361284
 +
|Backward
|-
|-
-
|T01I0006
+
|[[T01I0006]]
|unknown substrate ABC transporter permease
|unknown substrate ABC transporter permease
|Mfl401
|Mfl401
|2897807
|2897807
-
|
+
|After base 462568
 +
|Forward
|-
|-
-
|T01I0007
+
|[[T01I0007]]
|unknown lipoprotein
|unknown lipoprotein
|Mfl158
|Mfl158
|2897972
|2897972
-
|
+
|After base 173763
 +
|Backward
|-
|-
-
|T01I0008
+
|[[T01I0008]]
-
|tRNA & rRNA cytosine-C5-methylase
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|tRNA & rRNA cytosine-C5-methylase; rrmb, sun, fmu gene, methylates 16S C967
-
|Mfl195
+
|Mfl196
-
|2898173
+
|2898042
-
|
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|After base 223810
 +
|Forward
|-
|-
-
|T01I0009
+
|[[T01I0009]]
-
|
+
|hypothetical protein
-
|
+
|Mfl627
-
|
+
|2897837
-
|
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|After base 734780
 +
|Backward
|-
|-
-
|T01I0010
+
|[[T01I0010]]
-
|S-adenosylmethionine:2-demethylmenaquinone methyltransferase
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|S-adenosylmethionine:2-demethylmenaquinone methyltransferase; probable horiz gene xfr; reversed, no CGR codons, no mollicute homolog
|Mfl078
|Mfl078
|2898239
|2898239
-
|
+
|After base 100177
 +
|Backward
|-
|-
-
|T01I0011
+
|[[T01I0011]]
|unknown substrate PTS system IIABC component
|unknown substrate PTS system IIABC component
|Mfl619
|Mfl619
|2897756
|2897756
-
|
+
|After base 727130
 +
|Backward
|-
|-
-
|T01I0012
+
|[[T01I0012]]
-
|membrane-associated lipoprotein precurser
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|membrane-associated lipoprotein precursor
|Mfl446
|Mfl446
|2897883
|2897883
-
|
+
|After base 524992
 +
|Backward
|-
|-
-
|T01I0013
+
|[[T01I0013]]
-
|
+
|DNA uptake protein
-
|
+
|Mfl369
-
|
+
|2898020
-
|
+
|After base 430323
 +
|Backward
 +
|-
 +
|[[T01I0014]]
 +
|unknown transmembrane protein
 +
|Mfl518
 +
|2897757
 +
|After base 610629
 +
|Forward
 +
|-
 +
|[[T01I0017]]
 +
|unknown lipoprotein
 +
|Mfl158
 +
|2897972
 +
|After base 175541
 +
|Backward
 +
|-
 +
|[[T01I0018]]
 +
|methylenetetrahydrofolate dehydrogenase; essential in MG (MG013)
 +
|Mfl167
 +
|2898248
 +
|After base 190467
 +
|Backward
 +
|-
 +
|[[T01I0019]]
 +
|unknown transmembrane protein
 +
|Mfl326
 +
|2897843
 +
|After base 384336
 +
|Forward
 +
|-
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|T01I0147
 +
|putative amino acid ABC transporter permease component
 +
|Mfl184
 +
|2897621
 +
|After base 215665
 +
|Forward
 +
|-
 +
|T01I0149
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|sucrose-6-phosphate hydrolase
 +
|Mfl515
 +
|2897729
 +
|After base  607393
 +
|Forward
 +
|-
 +
|T01I0150
 +
|beta-glucosidase
 +
|Mfl425
 +
|2898197
 +
|After base 498791
 +
|Forward
|}
|}
[[Category:Mesoplasma florum]] [[Category:In silico]]
[[Category:Mesoplasma florum]] [[Category:In silico]]

Current revision

Spreadsheet with Data

Image:MF Transposon Insertions.xls

Image:TI-287-383.xls

Image:TI-601-696.xls

Insertion locations sorted and genes knocked out identified: Image:MF-transposon-insertions.xls


Sequencing results for transposon insertion events

Mutant Disrupted Gene Gene's Locus Tag Gene ID Exact Insertion Site Orientation of Transposon

In relation to the direction of the genome, not of the gene

T01I0001 sucrose PTS system IIBC component Mfl516 2897720 After base 608314 Backward
T01I0002 beta-glucosidase Mfl011 2898258 After base 15546 Backward
T01I0003 sucrose PTS system IIBC component Mfl516 2897720 After base 608314 Backward
T01I0004 unknown substrate ABC transporter permease component Mfl159 2898241 After base 179139 Forward
T01I0005 putative transcriptional regulator Mfl311 2897948 After base 361284 Backward
T01I0006 unknown substrate ABC transporter permease Mfl401 2897807 After base 462568 Forward
T01I0007 unknown lipoprotein Mfl158 2897972 After base 173763 Backward
T01I0008 tRNA & rRNA cytosine-C5-methylase; rrmb, sun, fmu gene, methylates 16S C967 Mfl196 2898042 After base 223810 Forward
T01I0009 hypothetical protein Mfl627 2897837 After base 734780 Backward
T01I0010 S-adenosylmethionine:2-demethylmenaquinone methyltransferase; probable horiz gene xfr; reversed, no CGR codons, no mollicute homolog Mfl078 2898239 After base 100177 Backward
T01I0011 unknown substrate PTS system IIABC component Mfl619 2897756 After base 727130 Backward
T01I0012 membrane-associated lipoprotein precursor Mfl446 2897883 After base 524992 Backward
T01I0013 DNA uptake protein Mfl369 2898020 After base 430323 Backward
T01I0014 unknown transmembrane protein Mfl518 2897757 After base 610629 Forward
T01I0017 unknown lipoprotein Mfl158 2897972 After base 175541 Backward
T01I0018 methylenetetrahydrofolate dehydrogenase; essential in MG (MG013) Mfl167 2898248 After base 190467 Backward
T01I0019 unknown transmembrane protein Mfl326 2897843 After base 384336 Forward
T01I0147 putative amino acid ABC transporter permease component Mfl184 2897621 After base 215665 Forward
T01I0149 sucrose-6-phosphate hydrolase Mfl515 2897729 After base 607393 Forward
T01I0150 beta-glucosidase Mfl425 2898197 After base 498791 Forward
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