Mesoplasma florum:Sequence Analysis
From OpenWetWare
(Difference between revisions)
m (→Sequencing results for transposon insertion events) |
Current revision (18:58, 15 May 2008) (view source) |
||
(21 intermediate revisions not shown.) | |||
Line 1: | Line 1: | ||
+ | Spreadsheet with Data | ||
+ | |||
+ | [[Image:MF Transposon Insertions.xls]] | ||
+ | |||
+ | [[Image:TI-287-383.xls]] | ||
+ | |||
+ | [[Image:TI-601-696.xls]] | ||
+ | |||
+ | Insertion locations sorted and genes knocked out identified: [[Image:MF-transposon-insertions.xls]] | ||
+ | |||
+ | |||
===Sequencing results for transposon insertion events=== | ===Sequencing results for transposon insertion events=== | ||
Line 7: | Line 18: | ||
!Gene ID | !Gene ID | ||
!width="115"|'''Exact Insertion Site''' | !width="115"|'''Exact Insertion Site''' | ||
- | |||
!Orientation of Transposon | !Orientation of Transposon | ||
+ | <sub>In relation to the direction of the genome, not of the gene</sub> | ||
|- | |- | ||
|[[T01I0001]] | |[[T01I0001]] | ||
Line 15: | Line 26: | ||
|2897720 | |2897720 | ||
|After base 608314 | |After base 608314 | ||
- | |||
|Backward | |Backward | ||
|- | |- | ||
Line 23: | Line 33: | ||
|2898258 | |2898258 | ||
|After base 15546 | |After base 15546 | ||
- | |||
|Backward | |Backward | ||
|- | |- | ||
Line 31: | Line 40: | ||
|2897720 | |2897720 | ||
|After base 608314 | |After base 608314 | ||
- | |||
|Backward | |Backward | ||
|- | |- | ||
Line 39: | Line 47: | ||
|2898241 | |2898241 | ||
|After base 179139 | |After base 179139 | ||
- | |||
|Forward | |Forward | ||
|- | |- | ||
Line 47: | Line 54: | ||
|2897948 | |2897948 | ||
|After base 361284 | |After base 361284 | ||
- | |||
|Backward | |Backward | ||
|- | |- | ||
Line 55: | Line 61: | ||
|2897807 | |2897807 | ||
|After base 462568 | |After base 462568 | ||
- | |||
|Forward | |Forward | ||
|- | |- | ||
Line 63: | Line 68: | ||
|2897972 | |2897972 | ||
|After base 173763 | |After base 173763 | ||
- | |||
|Backward | |Backward | ||
|- | |- | ||
|[[T01I0008]] | |[[T01I0008]] | ||
- | |tRNA & rRNA cytosine-C5-methylase | + | |tRNA & rRNA cytosine-C5-methylase; rrmb, sun, fmu gene, methylates 16S C967 |
|Mfl196 | |Mfl196 | ||
|2898042 | |2898042 | ||
|After base 223810 | |After base 223810 | ||
- | |||
|Forward | |Forward | ||
|- | |- | ||
Line 79: | Line 82: | ||
|2897837 | |2897837 | ||
|After base 734780 | |After base 734780 | ||
- | |||
|Backward | |Backward | ||
|- | |- | ||
|[[T01I0010]] | |[[T01I0010]] | ||
- | |S-adenosylmethionine:2-demethylmenaquinone methyltransferase | + | |S-adenosylmethionine:2-demethylmenaquinone methyltransferase; probable horiz gene xfr; reversed, no CGR codons, no mollicute homolog |
|Mfl078 | |Mfl078 | ||
|2898239 | |2898239 | ||
|After base 100177 | |After base 100177 | ||
- | |||
|Backward | |Backward | ||
|- | |- | ||
Line 95: | Line 96: | ||
|2897756 | |2897756 | ||
|After base 727130 | |After base 727130 | ||
- | |||
|Backward | |Backward | ||
|- | |- | ||
Line 103: | Line 103: | ||
|2897883 | |2897883 | ||
|After base 524992 | |After base 524992 | ||
- | |||
|Backward | |Backward | ||
|- | |- | ||
Line 111: | Line 110: | ||
|2898020 | |2898020 | ||
|After base 430323 | |After base 430323 | ||
- | |||
|Backward | |Backward | ||
|- | |- | ||
Line 119: | Line 117: | ||
|2897757 | |2897757 | ||
|After base 610629 | |After base 610629 | ||
- | | | + | |Forward |
+ | |- | ||
+ | |[[T01I0017]] | ||
+ | |unknown lipoprotein | ||
+ | |Mfl158 | ||
+ | |2897972 | ||
+ | |After base 175541 | ||
+ | |Backward | ||
+ | |- | ||
+ | |[[T01I0018]] | ||
+ | |methylenetetrahydrofolate dehydrogenase; essential in MG (MG013) | ||
+ | |Mfl167 | ||
+ | |2898248 | ||
+ | |After base 190467 | ||
+ | |Backward | ||
+ | |- | ||
+ | |[[T01I0019]] | ||
+ | |unknown transmembrane protein | ||
+ | |Mfl326 | ||
+ | |2897843 | ||
+ | |After base 384336 | ||
+ | |Forward | ||
+ | |- | ||
+ | |T01I0147 | ||
+ | |putative amino acid ABC transporter permease component | ||
+ | |Mfl184 | ||
+ | |2897621 | ||
+ | |After base 215665 | ||
+ | |Forward | ||
+ | |- | ||
+ | |T01I0149 | ||
+ | |sucrose-6-phosphate hydrolase | ||
+ | |Mfl515 | ||
+ | |2897729 | ||
+ | |After base 607393 | ||
+ | |Forward | ||
+ | |- | ||
+ | |T01I0150 | ||
+ | |beta-glucosidase | ||
+ | |Mfl425 | ||
+ | |2898197 | ||
+ | |After base 498791 | ||
|Forward | |Forward | ||
|} | |} |
Current revision
Spreadsheet with Data
Image:MF Transposon Insertions.xls
Insertion locations sorted and genes knocked out identified: Image:MF-transposon-insertions.xls
Sequencing results for transposon insertion events
Mutant | Disrupted Gene | Gene's Locus Tag | Gene ID | Exact Insertion Site | Orientation of Transposon
_{In relation to the direction of the genome, not of the gene} |
---|---|---|---|---|---|
T01I0001 | sucrose PTS system IIBC component | Mfl516 | 2897720 | After base 608314 | Backward |
T01I0002 | beta-glucosidase | Mfl011 | 2898258 | After base 15546 | Backward |
T01I0003 | sucrose PTS system IIBC component | Mfl516 | 2897720 | After base 608314 | Backward |
T01I0004 | unknown substrate ABC transporter permease component | Mfl159 | 2898241 | After base 179139 | Forward |
T01I0005 | putative transcriptional regulator | Mfl311 | 2897948 | After base 361284 | Backward |
T01I0006 | unknown substrate ABC transporter permease | Mfl401 | 2897807 | After base 462568 | Forward |
T01I0007 | unknown lipoprotein | Mfl158 | 2897972 | After base 173763 | Backward |
T01I0008 | tRNA & rRNA cytosine-C5-methylase; rrmb, sun, fmu gene, methylates 16S C967 | Mfl196 | 2898042 | After base 223810 | Forward |
T01I0009 | hypothetical protein | Mfl627 | 2897837 | After base 734780 | Backward |
T01I0010 | S-adenosylmethionine:2-demethylmenaquinone methyltransferase; probable horiz gene xfr; reversed, no CGR codons, no mollicute homolog | Mfl078 | 2898239 | After base 100177 | Backward |
T01I0011 | unknown substrate PTS system IIABC component | Mfl619 | 2897756 | After base 727130 | Backward |
T01I0012 | membrane-associated lipoprotein precursor | Mfl446 | 2897883 | After base 524992 | Backward |
T01I0013 | DNA uptake protein | Mfl369 | 2898020 | After base 430323 | Backward |
T01I0014 | unknown transmembrane protein | Mfl518 | 2897757 | After base 610629 | Forward |
T01I0017 | unknown lipoprotein | Mfl158 | 2897972 | After base 175541 | Backward |
T01I0018 | methylenetetrahydrofolate dehydrogenase; essential in MG (MG013) | Mfl167 | 2898248 | After base 190467 | Backward |
T01I0019 | unknown transmembrane protein | Mfl326 | 2897843 | After base 384336 | Forward |
T01I0147 | putative amino acid ABC transporter permease component | Mfl184 | 2897621 | After base 215665 | Forward |
T01I0149 | sucrose-6-phosphate hydrolase | Mfl515 | 2897729 | After base 607393 | Forward |
T01I0150 | beta-glucosidase | Mfl425 | 2898197 | After base 498791 | Forward |