Mesoplasma florum:Sequence Analysis

Sequencing results for transposon insertion events

Mutant	Disrupted Gene	Gene's Locus Tag	Gene ID	Exact Insertion Site	~Percent of Gene before Insertion	Orientation of Transposon
T01I0001	sucrose PTS system IIBC component	Mfl516	2897720	After base 608314	26%	Backward
T01I0002	beta-glucosidase	Mfl011	2898258	After base 15546	50%	Backward
T01I0003	sucrose PTS system IIBC component	Mfl516	2897720	After base 608314	26%	Backward
T01I0004	unknown substrate ABC transporter permease component	Mfl159	2898241	After base 179139	58%	Forward
T01I0005	putative transcriptional regulator	Mfl311	2897948	After base 361284	68%	Backward
T01I0006	unknown substrate ABC transporter permease	Mfl401	2897807	After base 462568	42%	Forward
T01I0007	unknown lipoprotein	Mfl158	2897972	After base 173763	16%	Backward
T01I0008	tRNA & rRNA cytosine-C5-methylase; rrmb, sun, fmu gene, methylates 16S C967	Mfl196	2898042	After base 223810	12%	Forward
T01I0009	hypothetical protein	Mfl627	2897837	After base 734780	34%	Backward
T01I0010	S-adenosylmethionine:2-demethylmenaquinone methyltransferase; probable horiz gene xfr; reversed, no CGR codons, no mollicute homolog	Mfl078	2898239	After base 100177	51%	Backward
T01I0011	unknown substrate PTS system IIABC component	Mfl619	2897756	After base 727130	91%	Backward
T01I0012	membrane-associated lipoprotein precursor	Mfl446	2897883	After base 524992	5%	Backward
T01I0013	DNA uptake protein	Mfl369	2898020	After base 430323	28%	Backward
T01I0014	unknown transmembrane protein	Mfl518	2897757	After base 610629	81%	Forward
T01I0015				After base
T01I0016				After base
T01I0017	unknown lipoprotein	Mfl158	2897972	After base 175541	87%	Backward
T01I0018	methylenetetrahydrofolate dehydrogenase; essential in MG (MG013)	Mfl167	2898248	After base 190467	59%	Backward
T01I0019	unknown transmembrane protein	Mfl326	2897843	After base 384336	93%	Forward