Mesoplasma florum:Tetracycline resistance

Mutations of 16S region conveying Tetracycline resistance

• G1058C PMID=9661007 (P. acne)
• G92T
• T93-
• C95G
• T96G
• G360A
• G741A
• A782C
• C883G
• A965G PMID=12435699, PMID=15901694
• G966T
• A967C
• G1026A
• C1033T
• T1136C
• C1167T
• A1299G
• C1293T

Alignment of E. coli 16S with M. florum 16S sequences

     gi|4899487 TAAGGAGGTG ATCCAACCGC AGGTTCCCCT AC-----GGT TACCTTGTTA
     gi|1079909 TCAGGAYGAA CGCTGGCGGC ATG-CCTAAT ACATGCAAGT CGAACGGAGG

  51 CGACTTCACC CCAGTCATGA ATCACAAAGT GGTAAGCGCC CTCCCGAAGG TTAAGCTACC
     TGCTTGCACC TCAGTGGCGA ACGGGTGAGT AACACGTATC ---------- -TAATCTACC

 111 TACT------ -----TCTTT TGCAACCCAC TCCCATGGTG TGACGGGCGG TGTGTACAAG
     TTCTAGCGGG GGATAACTTT TGGAAACGA- ----AAGGTA ATACCGCATG TGGATGTTAT

 171 ----GCCCGG GAACGTATTC ACCGTGGCAT TCTGATCCAC GATTACTAGC GATTCCGACT
     TATCGCATGA GAAAACATTC AAAGATCCGT TTGGAT-CAC TAGAAGATGA GGATGCGGCG

 231 TCATGG---- -AGTCGAGTT GCAGACTCCA ATCCGGACTA CGACGCAC-- ----TTTATG
     TATTAGCTAG TAGGCGGGGT AAAGGCCC-- ACCTAGGCGA TGATACGTAG CCGAACTGAG
  
 291 AGGTCCGCTT GCTCTCGCGA GGTC------ --------GC TTCTCTTTGT ATGCGCCATT
     AGGT-TGATC GGCCACATTG GGACTGAGAT ACGGCCCAGA CTCCTACGGG AGGCAGCA--
 
 351 GTAGCACGTG TGTAGCCCTG GTCGTAAGGG CCATGATGAC TTGACGTCAT CCCCACCTTC
     GTAGGGAATT TTTCACAATG GACGAAAG-- -TCTGATGAA GCAATGCCG- ---------- 
 
 411 CTCCAGTTTA TCACTGGCAG TCTCCTTTGA GTT------C CCGGCCG-GA CCGCTGGCAA
     --------CG TGAGTGATGA CGGCCTTCGG GTTGTAAAGC TCTGTTGTAA GGGAAGAAAA
 
 471 CAAAGGATAA GGGTTGCGCT CGTT---GCG GGACTTAACC CAACATTTCA CAACACGAGC
     CATAGGAGAG GAAATGCTCT TATCTTGACG GTACCTTACC AGA------A AGCCACG-GC
 
 531 TGACGACAGC CATGCAGCAC CTGTCTCACG GTTCCCGAAG GCACATTCTC ATCTCTGAAA
     TAACTATGTG CCAGCAGCCG CGGTAATACA TAGGTGGCAA GCGT------ -TATCCGGA-
  
 591 ACTTCCGTGG ATGTCAAGAC CAGGTAAGGT TCTTCGCGTT GCATCGAATT AAACCACATG
     --TTTATTGG GCGTATAGGG TGCGTAGGCG GTTTCGCA-A GTTTGAGGTT AAAGCCCGGA
 
 651 CTCCACCGCT TGTGCGGGCC CCCGTCAATT ----CATTTG AGTTTTAACC TTGCGGCCGT
     GCTCAACTCC GGTTCG---C CTTGAAAACT GCGGGACTAG AATATCAGAG AGGTAAGCGG
 
 711 ACTCCCCAGG CGGTCGACTT AACGCGTTAG C-TCCGGAAG CCACGCCTCA AGGGCACAAC
     AATTCCATGT GTAGCGGTAA AATGCGTAGA TATATGGAAG ---AACACCA GTGGCGAAAG
 
 771 CTCCAAGTCG AC--ATCGTT TACGGCGTG- ---GACTACC AGGGTATCTA ATCCTGTTTG
     CGGCTTACTG GCTGATTATT GACGCTGAGG CACGAAAGCG TGGGGAGCAA ATAGGATTAG
 
 831 CTCCCCACGC -TTTCGCACC -TGAGCGTC- AGT-CTTCGT CCAGGGGGCC GCCTTCGCCA
     ATACCCTAGT AGTCCACGCC GTAAACGTTG AGTACTAAGT ATTGGGGATT ACCTCAG---
 
 891 CCGGTATTCC TCCAGATCTC TACGCATTTC ACCGCTACAC CTG-GAATTC TACCCCCCTC
     -------TGC TGCAGCTA-- -ACGCA-TTA AGTACTCCGC CTGAGTAGTA TGCTCGCAAG
 
 951 TACGAGACTC AA---GCTTG CCAGTATCAG ATGCAGTTCC CAGGTTGAGC CCGGGGATTT
     AGTGAAACTC AAAGGAATTG ACGGGGACCC GCACAAGT-- --GGTGGAGC ATGTGGTTT-
 
1011 CACATCTGAC TTAACA---- -AACCGCCTG CGTGCGCTTT ACGCCCAGTA ATTCCGATTA
     --AATTCGAA GCAACACGAA GAAC--CTTA CCAGGGCTTG ACATACAGTG --------CA
 
1071 ACGCTTGCAC CCTCCGTATT ACCGCGGCTG CTGGCACGGA GTTAGCCGGT GCTTCTTCTG
     AAGCT----- --ACAGAGAT GTAGTGGAGG TTAACATTGA TACAGGTGGT GCAT------
 
1131 CGGGTAACGT CAATGAGCA- ---AAGGTAT TAACTTTACT CCCTTCCTCC CCGCTGAAAG
     -GGTTGTCGT CAGTTCGTGC CGTGAGGTGT TGGGTTAAGT CCC------- ----------
 
1191 TACTTTACAA CCCGAAGGCC TTCTTCATAC ACGCGGCATG GCTGCATCAG GCTTGCGCCC
     ------GCAA --CGAACGCA ACCCTTGT-- -CGTTAGTTA CTAACATTAA GTTGAGGACT
 
1251 ATTGTGCAAT ATTCCCCACT GCTGCCTCCC GTAGGA--GT CTGGACCGTG TC------TC
     CTAACGAGA- -----CTGCT AGTGTAAGCT AGAGGAAGGT GGGGATGACG TCAAATCATC

1311 AGTTCCAGTG TGGCT--GGT CATCCTCTCA GACCAGCTAG GGATCGTCGC CTTGGTGAGC
     ATGCCCCTTA TGTCCTGGGC TA------CA CACGTGCTA- CAATGGCCGA TACAAAGAGT

1371 CGTTACCCCA CCA--ACAAG CTAATCCCAT CTGGGCACAT CCGAT--GGC AAGAGGCCCG
     CGCAATCTCG CGAGGGGGAG CTAATCTCAA AAAGTCGGTC TCAGTTCGGA TTGAAGTCTG

1431 AAGGTCCCCC TCTTTGGTCT TGCGACATTA TGCGGTATTA GCTACCGTTT CCAGTAGTTA
     CAACTCGACT TCATGAAGCC GGAATCACTA ----GTAATC GCGA------ --ATCAGCTA

1491 TCCCCCTCCA TCAGGCAGCT TCCCAGACAT TACTCAC-CC GTCCGCCACT C-------GT
     TGTC---GCG GTGAATACGT TCTCGGGTCT TGTACACACC GCCCGTCAAA CCACGAGAGT

1551 CAGCGA-AGC AGCAAGCTGC TTCCTGTTAC CGTTCGACTT GCATGT---- -GTTAGGCCT
     TGGTAATACC AGAAGTACGT TTCCTA--AC CGTAAGGAAG GCGCGTCCCA AGGTAGGACT

1611 GCCGCCAGCG TTCAATCTGA GCCATGAT-- ---------- -----CAAAC TCTTCAATTT
     AGCGATTGGG GTTAAGTCGT AACAAGGTAT CCGTACGGGA ACGTGCGGAT GGATCAC---

Canonical numbering of 16S ribosomal RNA sequence is keyed off of the E. coli sequence, given here in FASTA format and in Genbank format. This sequence is from Genbank U00096, bases 223771-225312.

>gi|48994873:223771-225312 Escherichia coli K12 MG1655, complete genome
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGT
AACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGAT
GGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTC
GGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGAC
GATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAG
GCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCT
TCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCC
GCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAA
TTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAA
CTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCG
TAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACGAAGACTGACGCTCAGGTGCGAAAGC
GTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGTTGTGCC
CTTGAGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAAC
TCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACC
TTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTG
CTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCT
TTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATG
ACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCG
ACCTCGCGAGAGCAAGCGGACCTCATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCAT
GAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACC
GCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACT
TTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCT
TA

       1 aaattgaaga gtttgatcat ggctcagatt gaacgctggc ggcaggccta acacatgcaa
      61 gtcgaacggt aacaggaaga agcttgcttc tttgctgacg agtggcggac gggtgagtaa
     121 tgtctgggaa actgcctgat ggagggggat aactactgga aacggtagct aataccgcat
     181 aacgtcgcaa gaccaaagag ggggaccttc gggcctcttg ccatcggatg tgcccagatg
     241 ggattagcta gtaggtgggg taacggctca cctaggcgac gatccctagc tggtctgaga
     301 ggatgaccag ccacactgga actgagacac ggtccagact cctacgggag gcagcagtgg
     361 ggaatattgc acaatgggcg caagcctgat gcagccatgc cgcgtgtatg aagaaggcct
     421 tcgggttgta aagtactttc agcggggagg aagggagtaa agttaatacc tttgctcatt
     481 gacgttaccc gcagaagaag caccggctaa ctccgtgcca gcagccgcgg taatacggag
     541 ggtgcaagcg ttaatcggaa ttactgggcg taaagcgcac gcaggcggtt tgttaagtca
     601 gatgtgaaat ccccgggctc aacctgggaa ctgcatctga tactggcaag cttgagtctc
     661 gtagaggggg gtagaattcc aggtgtagcg gtgaaatgcg tagagatctg gaggaatacc
     721 ggtggcgaag gcggccccct ggacgaagac tgacgctcag gtgcgaaagc gtggggagca
     781 aacaggatta gataccctgg tagtccacgc cgtaaacgat gtcgacttgg aggttgtgcc
     841 cttgaggcgt ggcttccgga gctaacgcgt taagtcgacc gcctggggag tacggccgca
     901 aggttaaaac tcaaatgaat tgacgggggc ccgcacaagc ggtggagcat gtggtttaat
     961 tcgatgcaac gcgaagaacc ttacctggtc ttgacatcca cagaactttc cagagatgga
    1021 ttggtgcctt cgggaactgt gagacaggtg ctgcatggct gtcgtcagct cgtgttgtga
    1081 aatgttgggt taagtcccgc aacgagcgca acccttatct tttgttgcca gcggtccggc
    1141 cgggaactca aaggagactg ccagtgataa actggaggaa ggtggggatg acgtcaagtc
    1201 atcatggccc ttacgaccag ggctacacac gtgctacaat ggcgcataca aagagaagcg
    1261 acctcgcgag agcaagcgga cctcataaag tgcgtcgtag tccggattgg agtctgcaac
    1321 tcgactccat gaagtcggaa tcgctagtaa tcgtggatca gaatgccacg gtgaatacgt
    1381 tcccgggcct tgtacacacc gcccgtcaca ccatgggagt gggttgcaaa agaagtaggt
    1441 agcttaacct tcgggagggc gcttaccact ttgtgattca tgactggggt gaagtcgtaa
    1501 caaggtaacc gtaggggaac ctgcggttgg atcacctcct ta