Mesoplasma florum:Transposome construction
- Cut the transposome out of the containing plasmid with PvuII enzyme, which cuts at the correct location at the mosaic end.
- Cut also with an enzyme which produces shorter fragments from the remaining plasmid backbone to make gel purification easier. Sau3AI is a good enzyme.
- Use NEB Buffer 1 with BSA which is good for both Sau3AI and PvuII.
- TT01 transposon is 2478 bp long