Mesoplasma florum:Transposome construction

From OpenWetWare

Revision as of 15:07, 1 October 2007 by Tk (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search
  • Cut the transposome out of the containing plasmid with PvuII enzyme, which cuts at the correct location at the mosaic end.
  • Cut also with an enzyme which produces shorter fragments from the remaining plasmid backbone to make gel purification easier. Sau3AI is a good enzyme.
    • Use NEB Buffer 1 with BSA which is good for both Sau3AI and PvuII.
  • TT01 transposon is 2478 bp long
Personal tools