# Methods to determine the size of an object in microns

### From OpenWetWare

(Difference between revisions)

(→=Mathematically) |
|||

Line 1: | Line 1: | ||

- | ===Mathematically== | + | ===Mathematically=== |

- | Pixels on our CCD are 6.45um. | + | Pixels on our CCD are 6.45um. The size (in microns) of one pixel in an image will depend on how much the image was magnified before reaching the CCD: |

- | + | ||

- | The size (in microns) of one pixel in an image will depend on how much the image was magnified before reaching the CCD: | + | |

<math>\mbox{Pixel size} = \frac{6.45um}{\mbox{total magnification}}</math> | <math>\mbox{Pixel size} = \frac{6.45um}{\mbox{total magnification}}</math> | ||

+ | |||

+ | Assuming that the magnifier is at position 1x (not 1.5x), this formula gives us: | ||

+ | |||

+ | 60x : 0.1075 um/pixel or 9.30 pixels/um | ||

+ | 40x : 0.1613 um/pixel or 6.21 pixels/um | ||

+ | 20x : 0.3225 um/pixel or 3.10 pixels/um | ||

+ | 10x : 0.645 um/pixel or 1.55 pixel/um |

## Revision as of 13:42, 11 August 2005

### Mathematically

Pixels on our CCD are 6.45um. The size (in microns) of one pixel in an image will depend on how much the image was magnified before reaching the CCD:

Assuming that the magnifier is at position 1x (not 1.5x), this formula gives us:

60x : 0.1075 um/pixel or 9.30 pixels/um 40x : 0.1613 um/pixel or 6.21 pixels/um 20x : 0.3225 um/pixel or 3.10 pixels/um 10x : 0.645 um/pixel or 1.55 pixel/um