Mimulus:DNA Extraction Protocol - Revision history

CarrieWu at 14:30, 22 February 2007

CarrieWu — Thu, 22 Feb 2007 14:30:15 GMT

←Older revision		Revision as of 14:30, 22 February 2007
Line 58:		Line 58:


-	CTAB Leaf Buffer (Recipe to make 100 uL Buffer)	+	CTAB Leaf Buffer (Recipe to make 100 mL Buffer)
	*1 M Tris Buffer, pH 8.0 10 mL		*1 M Tris Buffer, pH 8.0 10 mL
	*NaCl 8.3 g		*NaCl 8.3 g

Skosuri — Tue, 05 Dec 2006 18:54:02 GMT

←Older revision		Revision as of 18:54, 5 December 2006
Line 1:		Line 1:
-	Return to [[Mimulus Community]]	+	Return to [[Mimulus\|Mimulus Community]]

	Here is the protocol that is used in the Willis lab:		Here is the protocol that is used in the Willis lab:

Skosuri — Tue, 05 Dec 2006 18:52:55 GMT

←Older revision

Revision as of 18:52, 5 December 2006

Lowry — Sun, 02 Apr 2006 19:13:51 GMT

←Older revision		Revision as of 19:13, 2 April 2006
Line 1:		Line 1:
-	~~Here is the protocol that is used in the Willis lab:~~
-
	Return to [[Mimulus Community]]		Return to [[Mimulus Community]]
		+
		+	Here is the protocol that is used in the Willis lab:

	Mimulus Corolla (Leaf) DNA Prep:		Mimulus Corolla (Leaf) DNA Prep:

Lowry — Sun, 02 Apr 2006 19:13:11 GMT

←Older revision		Revision as of 19:13, 2 April 2006
Line 6:		Line 6:

	Things to do first:		Things to do first:
		+
	1) Turn on water bath (60 degrees C).		1) Turn on water bath (60 degrees C).
		+
	2) Get liquid nitrogen.		2) Get liquid nitrogen.
		+
	3) Get sample tube/plates from -80 degree freezer. Keep samples chilled on ice.		3) Get sample tube/plates from -80 degree freezer. Keep samples chilled on ice.
		+
	4) Add one bead to each tube.		4) Add one bead to each tube.

Lowry — Sun, 02 Apr 2006 19:12:32 GMT

←Older revision		Revision as of 19:12, 2 April 2006
Line 1:		Line 1:
	Here is the protocol that is used in the Willis lab:		Here is the protocol that is used in the Willis lab:
		+
	Return to [[Mimulus Community]]		Return to [[Mimulus Community]]

Lowry — Sun, 02 Apr 2006 19:12:13 GMT

←Older revision		Revision as of 19:12, 2 April 2006
Line 1:		Line 1:
	Here is the protocol that is used in the Willis lab:		Here is the protocol that is used in the Willis lab:
-	Return to [Mimulus Community]	+	Return to [[Mimulus Community]]
-	[Mimulus Corolla (Leaf) DNA Prep]	+
		+	Mimulus Corolla (Leaf) DNA Prep:

	Things to do first:		Things to do first:

Lowry — Sun, 02 Apr 2006 19:11:00 GMT

←Older revision		Revision as of 19:11, 2 April 2006
Line 1:		Line 1:
	Here is the protocol that is used in the Willis lab:		Here is the protocol that is used in the Willis lab:
-		+	Return to [Mimulus Community]
	[Mimulus Corolla (Leaf) DNA Prep]		[Mimulus Corolla (Leaf) DNA Prep]

Lowry — Sun, 02 Apr 2006 19:10:18 GMT

←Older revision		Revision as of 19:10, 2 April 2006
Line 1:		Line 1:
	Here is the protocol that is used in the Willis lab:		Here is the protocol that is used in the Willis lab:
-	Mimulus Corolla (Leaf) DNA Prep	+
		+	[Mimulus Corolla (Leaf) DNA Prep]

	Things to do first:		Things to do first:

Lowry — Sun, 02 Apr 2006 19:09:38 GMT

←Older revision		Revision as of 19:09, 2 April 2006
Line 52:		Line 52:

	CTAB Leaf Buffer (Recipe to make 100 uL Buffer)		CTAB Leaf Buffer (Recipe to make 100 uL Buffer)
-	*1 M Tris Buffer, pH 8.0 10 mL	+	*1 M Tris Buffer, pH 8.0 10 mL
-	*NaCl 8.3 g	+	*NaCl 8.3 g
-	*EDTA 0.744 g	+	*EDTA 0.744 g
-	*CTAB 2 g	+	*CTAB 2 g
-	*Polyvinyl-Pyrrolidone (PVP) 2 g	+	*Polyvinyl-Pyrrolidone (PVP) 2 g
-	*Asorbic acid 0.088 g	+	*Asorbic acid 0.088 g


	Needed for Extraction:		Needed for Extraction:
-	*Genogrinder	+	*Genogrinder
-	*Centrifuge (Plate Centrifuge if you are using plates)	+	*Centrifuge (Plate Centrifuge if you are using plates)
-	*B-mercaptoethanol	+	*B-mercaptoethanol
-	*Isopropanol	+	*Isopropanol
-	*70% Ethanol	+	*70% Ethanol
-	*Chloroform	+	*Chloroform
-	*Liquid Nitrogen	+	*Liquid Nitrogen
-	*Fume Hood	+	*Fume Hood
-	*CTAB Leaf Buffer	+	*CTAB Leaf Buffer
-	*Water Bath	+	*Water Bath
-	*Incubator (optional)	+	*Incubator (optional)