http://www.openwetware.org/index.php?title=Mimulus:Microsatellite_recipes&action=history Revision history for this page on the wiki en MediaWiki 1.13.2 Thu, 20 Jun 2013 05:12:50 GMT http://www.openwetware.org/index.php?title=Mimulus:Microsatellite_recipes&diff=91424&oldid=prev <p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 04:37, 13 December 2006</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 5:</td> <td colspan="2" class="diff-lineno">Line 5:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*In addition to these recipes, check out the [[PCR Program for Microsatellites]].&nbsp; &nbsp;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*In addition to these recipes, check out the [[PCR Program for Microsatellites]].&nbsp; &nbsp;</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Contact David Lowry (dbl2@duke.edu) or other members of the [[Mimulus Community]] for more inforamtion about these markers.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Contact David Lowry (dbl2@duke.edu) or other members of the [[Mimulus Community]] for more inforamtion about these markers.</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*[[Media:Microsatellite_PCR_conditions.xls|Microsat conditions spreadsheet]]</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">==</del>Below are the recipes that have been fine-tuned for ''Mimulus guttatus''<del class="diffchange diffchange-inline">==</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Below are the recipes that have been fine-tuned for ''Mimulus guttatus''</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">[[Media:Microsatellite_PCR_conditions.xls|Microsat conditions spreadsheet]]</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==AAT 9==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==AAT 9==</div></td></tr> <!-- diff generator: internal 2013-06-20 05:12:51 --> </table> Wed, 13 Dec 2006 04:37:29 GMT Lowry http://www.openwetware.org/wiki/Talk:Mimulus:Microsatellite_recipes http://www.openwetware.org/index.php?title=Mimulus:Microsatellite_recipes&diff=91423&oldid=prev <p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 04:36, 13 December 2006</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 6:</td> <td colspan="2" class="diff-lineno">Line 6:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Contact David Lowry (dbl2@duke.edu) or other members of the [[Mimulus Community]] for more inforamtion about these markers.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Contact David Lowry (dbl2@duke.edu) or other members of the [[Mimulus Community]] for more inforamtion about these markers.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Below are the recipes that have been fine-tuned for ''Mimulus guttatus'':</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">==</ins>Below are the recipes that have been fine-tuned for ''Mimulus guttatus''<ins class="diffchange diffchange-inline">==</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[Media</ins>:<ins class="diffchange diffchange-inline">Microsatellite_PCR_conditions.xls|Microsat conditions spreadsheet]]</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==AAT 9==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==AAT 9==</div></td></tr> <!-- diff generator: internal 2013-06-20 05:12:51 --> </table> Wed, 13 Dec 2006 04:36:18 GMT Lowry http://www.openwetware.org/wiki/Talk:Mimulus:Microsatellite_recipes http://www.openwetware.org/index.php?title=Mimulus:Microsatellite_recipes&diff=90326&oldid=prev <p><a href="/wiki/Microsatellite_recipes" class="mw-redirect" title="Microsatellite recipes">Microsatellite recipes</a> moved to <a href="/wiki/Mimulus:Microsatellite_recipes" title="Mimulus:Microsatellite recipes">Mimulus:Microsatellite recipes</a></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 18:53, 5 December 2006</td> </tr> <!-- diff generator: internal 2013-06-20 05:12:51 --> </table> Tue, 05 Dec 2006 18:53:13 GMT Skosuri http://www.openwetware.org/wiki/Talk:Mimulus:Microsatellite_recipes http://www.openwetware.org/index.php?title=Mimulus:Microsatellite_recipes&diff=36250&oldid=prev <p><span class="autocomment">AAT 356</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 19:27, 8 May 2006</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 372:</td> <td colspan="2" class="diff-lineno">Line 372:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Cycles: 25</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Cycles: 25</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*H20 <del class="diffchange diffchange-inline">5</del>.05μl </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*H20 <ins class="diffchange diffchange-inline">8</ins>.05μl </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*10X Buffer 1.5μl </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*10X Buffer 1.5μl </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*MgCl2 (25mM) 1.2μl </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*MgCl2 (25mM) 1.2μl </div></td></tr> <!-- diff generator: internal 2013-06-20 05:12:51 --> </table> Mon, 08 May 2006 19:27:13 GMT Lowry http://www.openwetware.org/wiki/Talk:Mimulus:Microsatellite_recipes http://www.openwetware.org/index.php?title=Mimulus:Microsatellite_recipes&diff=36249&oldid=prev <p><span class="autocomment">AAT 278</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 19:26, 8 May 2006</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 240:</td> <td colspan="2" class="diff-lineno">Line 240:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*H20 <del class="diffchange diffchange-inline">2</del>.95μl </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*H20 <ins class="diffchange diffchange-inline">5</ins>.95μl </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*10X Buffer 1.5μl </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*10X Buffer 1.5μl </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*MgCl2 (25mM) 1.8μl </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*MgCl2 (25mM) 1.8μl </div></td></tr> <!-- diff generator: internal 2013-06-20 05:12:51 --> </table> Mon, 08 May 2006 19:26:37 GMT Lowry http://www.openwetware.org/wiki/Talk:Mimulus:Microsatellite_recipes http://www.openwetware.org/index.php?title=Mimulus:Microsatellite_recipes&diff=36248&oldid=prev <p><span class="autocomment">AAT 217</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 19:25, 8 May 2006</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 62:</td> <td colspan="2" class="diff-lineno">Line 62:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*H20 <del class="diffchange diffchange-inline">5</del>.05μl</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*H20 <ins class="diffchange diffchange-inline">8</ins>.05μl</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*10X Buffer 1.5μl</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*10X Buffer 1.5μl</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*MgCl2 (25mM) 1.2μl</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*MgCl2 (25mM) 1.2μl</div></td></tr> <tr><td colspan="2" class="diff-lineno">Line 68:</td> <td colspan="2" class="diff-lineno">Line 68:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*217R 0.6μl</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*217R 0.6μl</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*217F 0.25μl</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*217F 0.25μl</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Taq 0.2μl <del class="diffchange diffchange-inline"> </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Taq 0.2μl</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>&#160;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==AAT 222==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==AAT 222==</div></td></tr> <!-- diff generator: internal 2013-06-20 05:12:51 --> </table> Mon, 08 May 2006 19:25:08 GMT Lowry http://www.openwetware.org/wiki/Talk:Mimulus:Microsatellite_recipes http://www.openwetware.org/index.php?title=Mimulus:Microsatellite_recipes&diff=36247&oldid=prev <p><span class="autocomment">AAT 267</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 19:24, 8 May 2006</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 203:</td> <td colspan="2" class="diff-lineno">Line 203:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Annealing Temp: 49</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Annealing Temp: 49</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Cycles: 30</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Cycles: 30</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <!-- diff generator: internal 2013-06-20 05:12:51 --> </table> Mon, 08 May 2006 19:24:41 GMT Lowry http://www.openwetware.org/wiki/Talk:Mimulus:Microsatellite_recipes http://www.openwetware.org/index.php?title=Mimulus:Microsatellite_recipes&diff=36246&oldid=prev <p><span class="autocomment">AAT 267</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 19:24, 8 May 2006</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 202:</td> <td colspan="2" class="diff-lineno">Line 202:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Annealing Temp 49</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Annealing Temp<ins class="diffchange diffchange-inline">: </ins> 49</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Cycles: 30</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*H20 <del class="diffchange diffchange-inline">3</del>.85μl </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*H20 <ins class="diffchange diffchange-inline">6</ins>.85μl </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*10X Buffer 1.5μl </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*10X Buffer 1.5μl </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*MgCl2 (25mM) 2.4μl </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*MgCl2 (25mM) 2.4μl </div></td></tr> <!-- diff generator: internal 2013-06-20 05:12:51 --> </table> Mon, 08 May 2006 19:24:14 GMT Lowry http://www.openwetware.org/wiki/Talk:Mimulus:Microsatellite_recipes http://www.openwetware.org/index.php?title=Mimulus:Microsatellite_recipes&diff=36245&oldid=prev <p><span class="autocomment">AAT 300</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 19:23, 8 May 2006</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 290:</td> <td colspan="2" class="diff-lineno">Line 290:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*H20 <del class="diffchange diffchange-inline">5</del>.05μl </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*H20 <ins class="diffchange diffchange-inline">8</ins>.05μl </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*10X Buffer 1.5μl </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*10X Buffer 1.5μl </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*MgCl2 (25mM) 1.2μl </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*MgCl2 (25mM) 1.2μl </div></td></tr> <tr><td colspan="2" class="diff-lineno">Line 296:</td> <td colspan="2" class="diff-lineno">Line 296:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*300R 0.6μl </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*300R 0.6μl </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*300F 0.25μl </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*300F 0.25μl </div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Taq 0.2μl <del class="diffchange diffchange-inline"> </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Taq 0.2μl</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>&#160;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==AAT 308==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==AAT 308==</div></td></tr> <!-- diff generator: internal 2013-06-20 05:12:51 --> </table> Mon, 08 May 2006 19:23:25 GMT Lowry http://www.openwetware.org/wiki/Talk:Mimulus:Microsatellite_recipes http://www.openwetware.org/index.php?title=Mimulus:Microsatellite_recipes&diff=36244&oldid=prev <p><span class="autocomment">AAT 240</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 19:16, 8 May 2006</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 138:</td> <td colspan="2" class="diff-lineno">Line 138:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Cycles: 25</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Cycles: 25</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*H20 <del class="diffchange diffchange-inline">4</del>.45μl</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*H20 <ins class="diffchange diffchange-inline">7</ins>.45μl</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*10X Buffer 1.5μl</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*10X Buffer 1.5μl</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*MgCl2 (25mM) 1.8μl </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*MgCl2 (25mM) 1.8μl </div></td></tr> <tr><td colspan="2" class="diff-lineno">Line 144:</td> <td colspan="2" class="diff-lineno">Line 144:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*240R 0.6μl </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*240R 0.6μl </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*240F 0.25μl</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*240F 0.25μl</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Taq 0.2μl <del class="diffchange diffchange-inline"> </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Taq 0.2μl</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>&#160;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==AAT 242==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==AAT 242==</div></td></tr> <!-- diff generator: internal 2013-06-20 05:12:51 --> </table> Mon, 08 May 2006 19:16:43 GMT Lowry http://www.openwetware.org/wiki/Talk:Mimulus:Microsatellite_recipes