Misc: Difference between revisions

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Testing page designs and layouts.
Testing page designs and layouts.
<div style="padding: 2px">[[Image:OWW_Materials.jpg|784px]]</div>


<!------------------------This is so much fun. ----------------------------------->
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[[Image:OpenWetware ltbluegreen.jpg|780px]]
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<!------------------------------------------------------------------------------->
==Media==
<!------------------------Protocol News/Updates---------------------------------->
===Defined Media===
<!------------------------------------------------------------------------------->
*[[Neidhardt EZ Rich Defined]]
==Protocol News==
*[[M9 salts]]
&bull; <font face="courier new" size=1>00/00/00 </font>ABC Added <BR>
*[[M9 media]]
&bull; <font face="courier new" size=1>00/00/00 </font>XYZ Improved <BR>
*[[M9 supplemented media]]
*[[SC]]
*[[SD]]
*[[TSS]]
===Standard Media===
*[[LB]]
*[[SOC]]
*[[T broth]]
*[[YPD]]
*[[Silver: YEPD Liquid|YEPD]]
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<!------------------------------------------------------------------------------->
==Enzymes==
<!-----------------------------------In vivo------------------------------------->
===Phosphatases===
<!------------------------------------------------------------------------------->
*[[Antarctic Phosphatase]]
==''In vivo''==
*[[Alkaline Phosphatase]]
<showhide>
===RNA Polymerases===
{{size|2|'''''Escherichia coli'''''}}__HIDER__
*[[T7 RNA Polymerase]]
<hide>
*[[T4 RNA Polymerase]]
&bull; [[Bacterial cell culture|<font style="color:#000000">Bacterial Cell culture</font>]] <BR>
===DNA Polymerases===
&bull; [[Bacterial Transformation |<font style="color:#000000">Bacterial Transformation</font>]] <BR>
*[[Vent]]
&bull; [[Preparing chemically competent cells|<font style="color:#ffffff" size=1>Preparing chemically competent cells (TSS)</font>]] <BR>
*[[Taq]]
&bull; [[Transforming chemically competent cells|<font style="color:#ffffff" size=1>Transforming chemically competent cells (TSS)</font>]] <BR>
*[[Phusion]]
&bull; [[Electrocompetent cells|<font style="color:#ffffff" size=1>Preparing electrocompetent cells</font>]] <BR>
*[[Reverse Transcriptase|Reverse Transcriptase (RT) ]]
&bull; [[Electroporation|<font style="color:#ffffff" size=1>Transforming electrocompetent cells</font>]] <BR>
&bull; [[beta-Galactosidase Assay (better Miller)|<font style="color:#000000">&beta;-Galactosidase Assay</font>]] <font size=1>(A better Miller)</font> <BR>
&bull; [[Colony PCR|<font style="color:#000000">Colony PCR</font>]] <BR>
&bull; [[Making a long term stock of bacteria|<font style="color:#000000">Making long-term stock bacteria</font>]] <BR>
&bull; [[Miniprep|<font style="color:#000000">Miniprep</font>]] <BR>
&bull; [[P1vir phage transduction|<font style="color:#000000">P1vir phage transduction</font>]] <BR>
&bull; [[Pour plates|<font style="color:#000000">Pour LB plates</font>]] <BR>
&bull; [http://www.cf.ac.uk/biosi/staff/ehrmann/tools/factors.htm <font style="color:#000000">Protein expression resource</font>] <BR>
&bull; [[S_N Chemostat Protocol|<font style="color:#000000">S N Chemostat Protocol</font>]] <BR>
</hide>
</showhide>


<showhide>
===Restriction Enzymes===
{{size|2|'''''Mesoplasma florum'''''}}__HIDER__
*[[Inventory|Endy:Inventory]]
<hide>
&bull; [[Mesoplama florum:Culture media|<font style="color:#000000">Preparing culture media</font>]] <BR>
</hide>
</showhide>


<showhide>
{{size|2|'''T7'''}}__HIDER__
<hide>
&bull; [[Cesium Chloride Purification of T7|<font style="color:#000000">Purification T7: Cesium Chloride</font>]] <BR>
&bull; [[Studier Lysate Prep|<font style="color:#000000">Simple lysate protocol from plaque/lysate</font>]] <BR>
</hide>
</showhide>


<showhide>
</div>
{{size|2|'''Yeast'''}}__HIDER__
<hide>
&bull; [[Assaying mating|<font style="color:#000000">Assaying mating</font>]] <BR>
&bull; [[Cell Cycle Arrest|<font style="color:#000000">Cell Cycle Arrest</font>]] <BR>
&bull; [[Dropout plates for yeast|<font style="color:#000000">Dropout plates</font>]] <BR>
&bull; [[High Efficiency Transformation|<font style="color:#000000">High Efficiency Transformation</font>]] <BR>
&bull; [[Layered plates|<font style="color:#000000">Layering plates</font>]] <BR>
&bull; [[Pour YPD plates|<font style="color:#000000">Pour YPD plates</font>]] <BR>
&bull; [[Silver: Lysate for Western|<font style="color:#000000">Simple Yeast Lysate</font>]] <font size=1>(for Western etc.)</font> <BR>
&bull; [[Silver: Time-Lapse Microscopy|<font style="color:#000000">Time-Lapse Microscopy</font>]] <BR>
&bull; [[Yeast Colony PCR|<font style="color:#000000">Yeast Colony PCR</font>]] <BR>
&bull; [[Yeast DNA Prep|<font style="color:#000000">Yeast DNA Prep</font>]] <BR>


</hide>
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<!------------------------------------------------------------------------------->
<!-----------------------------------Empty Col----------------------------------->
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==Antibiotics==
*[[Ampicillin]]
*[[Apramycin]]
*[[Carbenicillin]]
*[[Chloramphenicol]]
*[[Erythromycin]]
*[[G418]]
*[[Gentamycin]]
*[[Hygromycin]]
*[[Kanamycin]]
*[[Nalidixic Acid]]
*[[Neomycin]]
*[[Phleomycin]]
*[[Puromycin]]
*[[Rifampicin]]
*[[Spectinomycin]]
*[[Streptomycin]]
*[[Tetracycline]]
*[[Trimethoprim]]
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<!------------------------------------------------------------------------------->
==Reporters==
<!-----------------------------------In silico----------------------------------->
===Dyes and stains===
<!------------------------------------------------------------------------------->
[[Dyes and stains | General information]]
==''In silico''==
*[[Acridine Orange]]
<showhide>
*[[Carboxyfluorescein Diacetate]]
{{size|2|'''Sequence Analysis'''}}__HIDER__
*[[DAPI]]
<hide>
*[[Fluorescein diacetate]]
&bull; [[BLAST|<font style="color:#000000">BLAST</font>]] <BR>
*[[Hoechst 33342]]
&bull; [[CLUSTALW|<font style="color:#000000">CLUSTALW</font>]] <BR>
*[[Propidium iodide]]
</hide>
*[[SYBR Green I]]
</showhide>
*[[SYBR Green II]]
 
*[[SYTO]]
<showhide>
===Fluorescent proteins===
{{size|2|'''Structure'''}}__HIDER__
*[[CFP]]
<hide>
*[[GFP]]
&bull; [[THREADER|<font style="color:#000000">THREADER</font>]] <BR>
*[[mRFP1]]
&bull; [[Homology Modelling|<font style="color:#000000">Homology Modeling</font>]] <BR>
*[[YFP]]
</hide>
</showhide>
 
<showhide>
{{size|2|'''Pathway Modelling'''}}__HIDER__
<hide>
&bull; [[FBA|<font style="color:#000000">Flux Balance Analysis</font>]] <BR>
&bull; [[MoMA|<font style="color:#000000">Minimisation of Metabolic Adjustment</font>]] <BR>
</hide>
</showhide>
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<!------------------------------------------------------------------------------->
==Agarose gel electrophoresis==
<!-----------------------------------In vitro------------------------------------>
*[[1X TAE]]
<!------------------------------------------------------------------------------->
*[[Loading dye]]
==''In vitro''==
*[[DNA ladder]]
<showhide>
*[[Ethidium Bromide]]
{{size|2|'''Nucleic acids'''}}__HIDER__
<hide>
&bull; [[Nucleic acid precipitation|<font style="color:#000000">Nucleic acid precipitation</font>]] <BR>
&bull; [[Phenol/chloroform extraction|<font style="color:#000000">Phenol/chloroform extraction</font>]] <BR>
</hide>
</showhide>
 
<showhide>
{{size|2|'''DNA'''}}__HIDER__
<hide>
&bull; [[Agarose gel electrophoresis|<font style="color:#000000">Agarose gel electrophoresis</font>]] <BR>
&bull; [[Annealing Primers|<font style="color:#000000">Annealing Primers</font>]] <BR>
&bull; [[Designing primers|<font style="color:#000000">Designing Primers</font>]] <BR>
&bull; [[DNA Ligation|<font style="color:#000000">DNA Ligation</font>]] <BR>
&bull; [[Library Generation|<font style="color:#000000">Library Generating</font>]] <BR>
&bull; [[Miniprep|<font style="color:#000000">Miniprep</font>]] <BR>
&bull; [[PCR|<font style="color:#000000">PCR</font>]] <BR>
&bull; [[Phosphatase treatment of linearized vector|<font style="color:#000000">Phosphate treatment linearized vector</font>]] <BR>
&bull; [http://arep.med.harvard.edu/polony/polony_protocols <font style="color:#000000">Polony Protocols</font>]<font size=1>(Church/Mitra Lab)</font> <BR>
&bull; [[PNK Treatment of DNA Ends|<font style="color:#000000">PNK Treatment of DNA ends</font>]] <BR>
&bull; [[Purification of DNA|<font style="color:#000000">Purification of DNA</font>]] <BR>
&bull; [[Reconstituting primers|<font style="color:#000000">Reconstituting Primers</font>]] <BR>
&bull; [[Restriction digest|<font style="color:#000000">Restriction Digest</font>]] <BR>
&bull; [['Round-the-horn site-directed mutagenesis|<font style="color:#000000">'Round-the-horn site-directed mutagenesis</font>]] <BR>
&bull; [[Sequencing DNA|<font style="color:#000000">Sequencing DNA</font>]] <BR>
&bull; [[Site-directed mutagenesis|<font style="color:#000000">Site-directed Mutagenesis</font>]] <BR>
&bull; [[Templiphi|<font style="color:#000000">Templiphi</font>]] <BR>
</hide>
</showhide>
 
<showhide>
{{size|2|'''RNA'''}}__HIDER__
<hide>
&bull; [[In vitro transcription with T7 RNA polymerase|<font style="color:#000000">In vitro transcription w/ T7 RNA polymerase</font>]] <BR>
&bull; [[Northern Blot, 32P End-Labeled Probes |<font style="color:#000000">Northern Blot, <sup>32</sup>P End-Labeled Probes</font>]] <BR>
&bull; [[Probe Prep, 32P End-Labeled Probes|<font style="color:#000000">Probe Prep, <sup>32</sup>P End-Labeled Probes</font>]] <BR>
&bull; [[RNA Extraction|<font style="color:#000000">RNA Extraction</font>]] <BR>
&bull; [[RNase Protection Assay|<font style="color:#000000">RNAse Protection Assay</font>]] <BR>
</hide>
</showhide>
 
<showhide>
{{size|2|'''Protein'''}}__HIDER__
<hide>
&bull; [[Acrylamide Gels|<font style="color:#000000">Acrylamide gels and SDS-PAGE</font>]] <BR>
&bull; [[Silver: Destain|<font style="color:#000000">Coomassie Destain</font>]] <BR>
&bull; [[Silver: Coomassie Stain|<font style="color:#000000">Coomassie Stain</font>]] <BR>
&bull; [[Keating:Experimental Protocols:HPLC|<font style="color:#000000">HPLC</font>]] <BR>
&bull; [[Silver staining|<font style="color:#000000">Silver staining</font>]] <BR>
&bull; [[Western Blot|<font style="color:#000000">Western Blot</font>]] <BR>
&bull; [[Purification of His-tagged proteins|<font style="color:#000000">Purification His-tagged proteins</font>]] <BR>
</hide>
</showhide>
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<!------------------------------------------------------------------------------->
==Miscellaneous==
<!-----------------------------------OTHER--------------------------------------->
*[[aTc]]
<!------------------------------------------------------------------------------->
*[[Arabinose]]
==Other==
*[[dNTPs]]
<showhide>
*[[IPTG]]
{{size|2|'''Flow Cytometry'''}}__HIDER__
*[[Silver: 10x PBS|10x PBS]]
<hide>
*[[ONPF]]
&bull; [[Flow Calibration|<font style="color:#000000">Flow Calibration</font>]] <BR>
*[[ProteinA_Sepharose Beads]]
</hide>
*[[Raffinose]]
</showhide>
 
<showhide>
{{size|2|'''Microfluidics'''}}__HIDER__
<hide>
&bull; [[Design a Microfluidic Chip|<font style="color:#000000">Design Microfluidic Chip</font>]] <BR>
&bull; [[Order a Microfluidic Chip from the Foundry|<font style="color:#000000">Order Microfluidic Chip from Foundry</font>]] <BR>
&bull; [[Operate Microfluidic Chemostat|<font style="color:#000000">Operate Microfluidic Chemostat</font>]] <BR>
</hide>
</showhide>
 
<showhide>
{{size|2|'''Microscopy'''}}__HIDER__
<hide>
&bull; [[Agarose Pads for Microscopy|<font style="color:#000000">Agarose Pads</font>]] <BR>
&bull; [[Quantitative Microscopy|<font style="color:#000000">Quantitative Microscopy</font>]] <BR>
&bull; [[Silver: Time-Lapse Microscopy|<font style="color:#000000">Time-Lapse Microscopy</font>]] <BR>
</hide>
</showhide>
 
<showhide>
{{size|2|'''Plate reader'''}}__HIDER__
<hide>
&bull; [[Endy:Victor3 plate reader|<font style="color:#000000">Perkin Elmer Victor<sup>3</sup></font>]]<font size=1><BR>Plate reader usage protocols</font> <BR>
</hide>
</showhide>


{{size|2|'''General resources'''}}<BR>
&bull; [http://protocol-online.org <font style="color:#000000">Protocol-online</font>] <font size=1><BR>Contains a lot of useful protocols and a popular discussion section.</font> <BR>
<!------------------------------------------------------------------------------->
<!-----------------------------------IN DEVELOPMENT------------------------------>
<!------------------------------------------------------------------------------->
{{size|2|'''Protocols in Development'''}}
'''Please Contribute!'''<BR>
&bull; [[Pulse-chase protein production|<font style="color:#000000">Pulse-chase protein production</font>]] <BR>
&bull; [[Fixing cells|<font style="color:#000000">Fixing cells</font>]] <BR>
&bull; [[B-galactosidase assay|<font style="color:#000000">&beta;-galactosidase assay</font>]] <BR>
&bull; [[RNA Half-life|<font style="color:#000000">RNA Half-life</font>]] <BR>
&bull; [[Denaturing acrylamide gel purification of nucleic acids|<font style="color:#000000">Denaturing acrylamide gel purification NA</font>]] <BR>
&bull; [[Quantification of nucleic acids|<font style="color:#000000">Quantification of nucleic acids</font>]] <BR>
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Revision as of 14:17, 26 February 2006

Testing page designs and layouts.

Agarose gel electrophoresis

<showhide> __SHOWALL__ __HIDEALL__ </showhide>


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