|
|
(54 intermediate revisions by 6 users not shown) |
Line 1: |
Line 1: |
| Testing main page formatting.
| | [[Image:MITeventsCalendar_subgroup_identification_idea.jpg|thumb|150px|right|so cute]] |
| <!------------------------This is so much fun. -----------------------------------> | | Unfinished set of icons that I've made that can be used for Community Portal subgroups.<BR> |
| | I need suggestions on... |
| | #What topics should be included as icons? |
| | #What icon best describes that topic? |
| | #Any other modifications (to the current icons or anything else). |
|
| |
|
| [[Image:OpenWetware ltbluegreen.jpg|780px]]
| |
|
| |
|
| {| cellspacing="1"
| |
| |- valign="top"
| |
| |rowspan="2" width="230px" style="border: 0px solid #000000; color: #000; background-color: #8CB143" valign="top"|
| |
| <div style="clear: right; text-align: left; float: left; padding: 0em .5em 0em">
| |
| ==Protocol News==
| |
| <showhide>__HIDER__<hide></hide></showhide>
| |
| </div>
| |
|
| |
|
| |width="188px" style="border: 0px solid #000000; color: #000; background-color: #6699CC" valign="top"|
| | This idea is borrowed from MIT TechTalk Event Highlights. |
| <div style="clear: right; text-align: left; float: left; padding: 0em .5em 0em">
| |
| ==''In vivo''==
| |
| <showhide>
| |
| {{size|2|'''''Escherichia coli'''''}}__HIDER__
| |
| <hide>
| |
| • [[Bacterial cell culture]] <BR>
| |
| • [[Bacterial Transformation | Bacterial transformation]] <BR>
| |
| :• [[Preparing chemically competent cells|Preparing chemically competent cells (TSS)]] <BR>
| |
| :• [[Transforming chemically competent cells|Transforming chemically competent cells (TSS)]] <BR>
| |
| :• [[Electrocompetent cells|Preparing electrocompetent cells]] <BR>
| |
| :• [[Electroporation|Transforming electrocompetent cells]] <BR>
| |
| • [[beta-Galactosidase Assay (A better Miller)|β-Galactosidase Assay (A better Miller)]] <BR>
| |
| • [[Colony PCR]] <BR>
| |
| • [[Making a long term stock of bacteria]] <BR>
| |
| • [[Miniprep]] <BR>
| |
| • [[P1vir phage transduction]] <BR>
| |
| • [[Pour plates|Pour LB plates]] <BR>
| |
| • [http://www.cf.ac.uk/biosi/staff/ehrmann/tools/factors.htm Protein expression resource] <BR>
| |
| • [[S_N Chemostat Protocol]] <BR>
| |
| </hide>
| |
| </showhide>
| |
|
| |
|
| <showhide>
| | [[Image:Courses.png|thumb|left|Courses]] |
| {{size|2|'''''Mesoplasma florum'''''}}__HIDER__
| | [[Image:Design.png|thumb|left|Design]] |
| <hide>
| | [[Image:Ideas.png|thumb|left|Ideas]] |
| • [[Mesoplama florum:Culture media|Preparing culture media]] <BR>
| | [[Image:InformationManagement.png|thumb|left|InfoMgt]] |
| </hide>
| | [[Image:Software.png|thumb|left|Software]] |
| </showhide>
| | [[Image:CommunityDevelopment.png|thumb|left|Community Dev]] |
|
| |
|
| <showhide>
| |
| {{size|2|'''T7'''}}__HIDER__
| |
| <hide>
| |
| • [[Cesium Chloride Purification of T7]] <BR>
| |
| • [[Studier Lysate Prep|Simple lysate protocol from plaque or lysate]] <BR>
| |
| </hide>
| |
| </showhide>
| |
|
| |
|
| <showhide>
| |
| {{size|2|'''Yeast'''}}__HIDER__
| |
| <hide>
| |
| • [[Assaying mating]] <BR>
| |
| • [[Cell Cycle Arrest]] <BR>
| |
| • [[Dropout plates for yeast]] <BR>
| |
| • [[High Efficiency Transformation]] <BR>
| |
| • [[Layered plates]] <BR>
| |
| • [[Pour YPD plates]] <BR>
| |
| • [[Silver: Lysate for Western|Simple Yeast Lysate]] (for Western etc.) <BR>
| |
| • [[Silver: Time-Lapse Microscopy|Time-Lapse Microscopy]] <BR>
| |
| • [[Yeast Colony PCR]] <BR>
| |
| • [[Yeast DNA Prep]] <BR>
| |
|
| |
|
| </hide>
| |
| </showhide>
| |
|
| |
|
| </div>
| |
|
| |
|
| |rowspan="2" width="184px" style="border: 0px solid #000000; color: #000; background-color: #5A87B6" valign="top"|
| |
| <div style="clear: right; text-align: left; float: left; padding: 0em .5em 0em">
| |
| ==''In silico''==
| |
| <showhide>
| |
| {{size|2|'''Sequence Analysis'''}}__HIDER__
| |
| <hide>
| |
| • [[BLAST]] <BR>
| |
| • [[CLUSTALW]] <BR>
| |
| </hide>
| |
| </showhide>
| |
|
| |
|
| <showhide>
| |
| {{size|2|'''Structure'''}}__HIDER__
| |
| <hide>
| |
| • [[THREADER]] <BR>
| |
| • [[Homology Modelling]] <BR>
| |
| </hide>
| |
| </showhide>
| |
|
| |
|
| <showhide>
| |
| {{size|2|'''Pathway Modelling'''}}__HIDER__
| |
| <hide>
| |
| • [[FBA|Flux Balance Analysis]] <BR>
| |
| • [[MoMA|Minimisation of Metabolic Adjustment]] <BR>
| |
| </hide>
| |
| </showhide>
| |
|
| |
|
| ==Other==
| |
| <showhide>
| |
| {{size|2|'''Flow Cytometry'''}}__HIDER__
| |
| <hide>
| |
| • [[Flow Calibration]] <BR>
| |
| </hide>
| |
| </showhide>
| |
|
| |
|
| <showhide>
| |
| {{size|2|'''Microfluidics'''}}__HIDER__
| |
| <hide>
| |
| • [[Design a Microfluidic Chip]] <BR>
| |
| • [[Order a Microfluidic Chip from the Foundry]] <BR>
| |
| • [[Operate Microfluidic Chemostat]] <BR>
| |
| </hide>
| |
| </showhide>
| |
|
| |
|
| <showhide>
| |
| {{size|2|'''Microscopy'''}}__HIDER__
| |
| <hide>
| |
| • [[Agarose Pads for Microscopy]] <BR>
| |
| • [[Quantitative Microscopy]] <BR>
| |
| • [[Silver: Time-Lapse Microscopy|Time-Lapse Microscopy]] <BR>
| |
| </hide>
| |
| </showhide>
| |
|
| |
|
| <showhide>
| | Test test test |
| {{size|2|'''Plate reader'''}}__HIDER__
| |
| <hide>
| |
| • [[Endy:Victor3 plate reader| Perkin Elmer Victor<sup>3</sup>]]<br>-Plate reader usage protocols <BR>
| |
| </hide>
| |
| </showhide>
| |
|
| |
|
| {{size|2|'''General resources'''}}<BR> | | {|style="text-align:left; background-color:#CDDE95; line-height: 1em" width="25%" cellspacing="0" cellpadding="0" |
| • [http://protocol-online.org <font style="color:#000000">Protocol-online</font>] <font size=1>Contains a lot of useful protocols and a popular discussion section.</font> <BR>
| | <!-----------Active Discussions -----------------> |
| | | |{{Click|image=Software.png|width=50px|height=50px|link=Roadmap}} |
| {{size|2|'''Protocols in Development'''}} | | |'''[[Roadmap|<font size=2>Development Roadmap</font>]]'''<br /><font size=1>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua.</font> |
| '''Please Contribute!'''<BR> | | <!----------------------------------------------> |
| • [[Pulse-chase protein production]] <BR>
| | |}<BR> |
| • [[Fixing cells]] <BR>
| | {|style="text-align:left; background-color:#DEE8B8; line-height: 1em" width="25%" cellspacing="0" cellpadding="0" |
| • [[B-galactosidase assay]] <BR>
| | <!-----------Active Discussions -----------------> |
| • [[RNA Half-life]] <BR>
| | |{{Click|image=Ideas.png|width=50px|height=50px|link=OpenWetWare:Ideas}} |
| • [[Denaturing acrylamide gel purification of nucleic acids]] <BR>
| | |'''[[OpenWetWare:Ideas|<font size=2>OWW Ideas</font>]]'''<br /><font size=1>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua.</font> |
| • [[Quantification of nucleic acids]] <BR>
| | <!----------------------------------------------> |
| </div> | | |}<BR> |
| | | {|style="text-align:left; background-color:#E5EDC8; line-height: 1em" width="25%" cellspacing="0" cellpadding="0" |
| |width="170px" style="border: 0px solid #000000; color: #000; background-color: #517AA3" valign="top"| | | <!-----------Active Discussions -----------------> |
| <div style="clear: right; text-align: left; float: left; padding: 0em .5em 0em"> | | |{{Click|image=Design.png|width=50px|height=50px|link=OpenWetWare:Logo and Promo Poster Discussion}} |
| ==Meta== | | |'''[[OpenWetWare:Logo and Promo Poster Discussion|<font size=2>Logo and Poster</font>]]'''<br /><font size=1>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua.</font> |
| • [[OpenWetWare:Jobs|<font style="color:#000000">Jobs at OpenWetWare</font>]]<BR>
| | <!----------------------------------------------> |
| • [[OpenWetWare:About|<font style="color:#000000">About OpenWetWare</font>]]<BR>
| | |}<BR> |
| • [[OpenWetWare steering committee|<font style="color:#000000">Steering Committee</font>]]<BR><BR>
| | {|style="text-align:left; background-color:#C5D0EA; line-height: 1em" width="25%" cellspacing="0" cellpadding="0" |
| • [[OpenWetware:Why join?|<font style="color:#000000">Why join?</font>]]<BR>
| | <!-----------Active Discussions -----------------> |
| • [[OpenWetWare:How_to_join|<font style="color:#000000">How to join</font>]]<BR>
| | |{{Click|image=InformationManagement.png|width=50px|height=50px|link=Information Management Discussion}} |
| • [[OpenWetWare:Getting started|<font style="color:#000000">'''Getting Started'''</font>]]<BR>
| | |'''[[Information Management Discussion|<font size=2>Info/Design Layouts</font>]]'''<br /><font size=1>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua.</font> |
| • [[OpenWetWare|<font style="color:#000000">Contributing</font>]]<BR>
| | <!----------------------------------------------> |
| • [[OpenWetWare:FAQ|<font style="color:#000000">Other FAQ</font>]]<BR><BR>
| | |}<BR> |
| • [[Etiquette|<font style="color:#000000">Etiquette</font>]]<BR>
| | {|style="text-align:left; background-color:#B4CE4E; line-height: 1em" width="25%" cellspacing="0" cellpadding="0" |
| • [[OpenWetWare:Tips|<font style="color:#000000">Tips</font>]]<BR>
| | <!-----------Active Discussions -----------------> |
| • [[OpenWetWare:Ideas|<font style="color:#000000">Thoughts on OWW</font>]]<BR>
| | |{{Click|image=CommunityDevelopment.png|width=50px|height=50px|link=Information Management Discussion}} |
| • [[Science 2.0|<font style="color:#000000">Science 2.0</font>]]<BR>
| | |'''[[Information Management Discussion|<font size=2>Info/Design Layouts</font>]]'''<br /><font size=1>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua.</font> |
| </div> | | <!----------------------------------------------> |
| |- | |
| | |
| |width="188px" style="border: 0px solid #000000; color: #000; background-color: #517AA3" valign="top"|
| |
| <div style="clear: right; text-align: left; float: left; padding: 0em .5em 0em">
| |
| ==''In vitro''==
| |
| <showhide> | |
| {{size|2|'''Nucleic acids'''}}__HIDER__ | |
| <hide>
| |
| • [[Nucleic acid precipitation]] <BR>
| |
| • [[Phenol/chloroform extraction]] <BR>
| |
| </hide>
| |
| </showhide>
| |
| | |
| <showhide>
| |
| {{size|2|'''DNA'''}}__HIDER__
| |
| <hide>
| |
| • [[Agarose gel electrophoresis]] <BR>
| |
| • [[Annealing Primers]] <BR>
| |
| • [[Designing primers]] <BR>
| |
| • [[DNA Ligation]] <BR>
| |
| • [[Library Generation]] <BR>
| |
| • [[Miniprep]] <BR>
| |
| • [[PCR]] <BR>
| |
| • [[Phosphatase treatment of linearized vector]] <BR>
| |
| • [http://arep.med.harvard.edu/polony/polony_protocols Polony Protocols (Church/Mitra Lab)] <BR>
| |
| • [[PNK Treatment of DNA Ends]] <BR>
| |
| • [[Purification of DNA]] <BR>
| |
| • [[Reconstituting primers]] <BR>
| |
| • [[Restriction digest]] <BR>
| |
| • [['Round-the-horn site-directed mutagenesis]] <BR>
| |
| • [[Sequencing DNA]] <BR>
| |
| • [[Site-directed mutagenesis]] <BR>
| |
| • [[Templiphi]] <BR>
| |
| </hide>
| |
| </showhide>
| |
| | |
| <showhide>
| |
| {{size|2|'''RNA'''}}__HIDER__
| |
| <hide>
| |
| • [[In vitro transcription with T7 RNA polymerase]] <BR>
| |
| • [[Northern Blot, 32P End-Labeled Probes |Northern Blot, <sup>32</sup>P End-Labeled Probes]] <BR>
| |
| • [[Probe Prep, 32P End-Labeled Probes|Probe Prep, <sup>32</sup>P End-Labeled Probes]] <BR>
| |
| • [[RNA Extraction]] <BR>
| |
| • [[RNase Protection Assay]] <BR>
| |
| </hide>
| |
| </showhide>
| |
| | |
| <showhide>
| |
| {{size|2|'''Protein'''}}__HIDER__
| |
| <hide>
| |
| • [[Acrylamide Gels|Acrylamide gels and SDS-PAGE]] <BR>
| |
| • [[Silver: Destain|Coomassie Destain]] <BR>
| |
| • [[Silver: Coomassie Stain|Coomassie Stain]] <BR>
| |
| • [[Keating:Experimental Protocols:HPLC|HPLC]] <BR>
| |
| • [[Silver staining]] <BR>
| |
| • [[Western Blot]] <BR>
| |
| • [[Purification of His-tagged proteins]] <BR>
| |
| </hide>
| |
| </showhide>
| |
| | |
| </div>
| |
| | |
| |width="170px" style="border: 0px solid #000000; color: #000; background-color: #6699CC" valign="top"|
| |
| <div style="clear: right; text-align: left; float: left; padding: 0em .5em 0em"> | |
| ==Discussion== | |
| • [[Logo and Poster Discussion|<font style="color:#000000">Logo and Poster</font>]]<BR>
| |
| • [[Information Management Discussion|<font style="color:#000000">Information Management</font>]]<BR>
| |
| • [[Software Development Discussion|<font style="color:#000000">Software Development</font>]]<BR>
| |
| </div> | |
| |} | | |} |
|
| |
| <showhide>
| |
| __SHOWALL__ __HIDEALL__
| |
| </showhide>
| |
|
| |
| __NOEDITSECTION__
| |
| __NOTOC__
| |