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| Testing page designs and layouts.
| | [[Image:MITeventsCalendar_subgroup_identification_idea.jpg|thumb|150px|right|so cute]] |
| | Unfinished set of icons that I've made that can be used for Community Portal subgroups.<BR> |
| | I need suggestions on... |
| | #What topics should be included as icons? |
| | #What icon best describes that topic? |
| | #Any other modifications (to the current icons or anything else). |
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| <!------------------------This is so much fun. ----------------------------------->
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| [[Image:OpenWetware ltbluegreen.jpg|780px]]
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| {| cellspacing="1"
| | This idea is borrowed from MIT TechTalk Event Highlights. |
| |- valign="top"
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| |rowspan="2" width="150px" style="border: 0px solid #000000; color: #000; background-color: #8CB143" valign="top"|
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| <div style="clear: right; text-align: left; float: left; padding: 0em .5em 0em">
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| ==Protocol News==
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| <showhide>__HIDER__<hide></hide></showhide>
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| </div>
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| |width="245px" style="border: 0px solid #000000; color: #000; background-color: #6699CC" valign="top"|
| | [[Image:Courses.png|thumb|left|Courses]] |
| <div style="clear: right; text-align: left; float: left; padding: 0em .5em 0em">
| | [[Image:Design.png|thumb|left|Design]] |
| ==''In vivo''==
| | [[Image:Ideas.png|thumb|left|Ideas]] |
| <showhide>
| | [[Image:InformationManagement.png|thumb|left|InfoMgt]] |
| {{size|2|'''''Escherichia coli'''''}}__HIDER__
| | [[Image:Software.png|thumb|left|Software]] |
| <hide>
| | [[Image:CommunityDevelopment.png|thumb|left|Community Dev]] |
| • [[Bacterial cell culture|<font style="color:#000000">Bacterial Cell culture</font>]] <BR>
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| • [[Bacterial Transformation |<font style="color:#000000">Bacterial Transformation</font>]] <BR>
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| • [[Preparing chemically competent cells|<font style="color:#ffffff" size=1>Preparing chemically competent cells (TSS)</font>]] <BR>
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| • [[Transforming chemically competent cells|<font style="color:#ffffff" size=1>Transforming chemically competent cells (TSS)</font>]] <BR>
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| • [[Electrocompetent cells|<font style="color:#ffffff" size=1>Preparing electrocompetent cells</font>]] <BR>
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| • [[Electroporation|<font style="color:#ffffff" size=1>Transforming electrocompetent cells</font>]] <BR>
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| • [[beta-Galactosidase Assay (better Miller)|<font style="color:#000000">β-Galactosidase Assay</font>]] <font size=1>(A better Miller)</font> <BR>
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| • [[Colony PCR|<font style="color:#000000">Colony PCR</font>]] <BR>
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| • [[Making a long term stock of bacteria|<font style="color:#000000">Making long-term stock bacteria</font>]] <BR>
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| • [[Miniprep|<font style="color:#000000">Miniprep</font>]] <BR>
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| • [[P1vir phage transduction|<font style="color:#000000">P1vir phage transduction</font>]] <BR>
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| • [[Pour plates|<font style="color:#000000">Pour LB plates</font>]] <BR>
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| • [http://www.cf.ac.uk/biosi/staff/ehrmann/tools/factors.htm <font style="color:#000000">Protein expression resource</font>] <BR>
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| • [[S_N Chemostat Protocol|<font style="color:#000000">S N Chemostat Protocol</font>]] <BR>
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| </hide>
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| </showhide>
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| <showhide>
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| {{size|2|'''''Mesoplasma florum'''''}}__HIDER__
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| <hide>
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| • [[Mesoplama florum:Culture media|<font style="color:#000000">Preparing culture media</font>]] <BR>
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| </hide>
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| </showhide>
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| <showhide>
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| {{size|2|'''T7'''}}__HIDER__
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| <hide>
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| • [[Cesium Chloride Purification of T7|<font style="color:#000000">Purification T7: Cesium Chloride</font>]] <BR>
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| • [[Studier Lysate Prep|<font style="color:#000000">Simple lysate protocol from plaque or lysate</font>]] <BR>
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| </hide>
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| </showhide>
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| <showhide>
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| {{size|2|'''Yeast'''}}__HIDER__
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| <hide>
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| • [[Assaying mating|<font style="color:#000000">Assaying mating</font>]] <BR>
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| • [[Cell Cycle Arrest|<font style="color:#000000">Cell Cycle Arrest</font>]] <BR>
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| • [[Dropout plates for yeast|<font style="color:#000000">Dropout plates</font>]] <BR>
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| • [[High Efficiency Transformation|<font style="color:#000000">High Efficiency Transformation</font>]] <BR>
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| • [[Layered plates|<font style="color:#000000">Layering plates</font>]] <BR>
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| • [[Pour YPD plates|<font style="color:#000000">Pour YPD plates</font>]] <BR>
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| • [[Silver: Lysate for Western|<font style="color:#000000">Simple Yeast Lysate</font>]] <font size=1>(for Western etc.)</font> <BR>
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| • [[Silver: Time-Lapse Microscopy|<font style="color:#000000">Time-Lapse Microscopy</font>]] <BR>
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| • [[Yeast Colony PCR|<font style="color:#000000">Yeast Colony PCR</font>]] <BR>
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| • [[Yeast DNA Prep|<font style="color:#000000">Yeast DNA Prep</font>]] <BR>
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| </hide>
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| </showhide>
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| </div>
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| |rowspan="2" width="184px" style="border: 0px solid #000000; color: #000; background-color: #5A87B6" valign="top"|
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| <div style="clear: right; text-align: left; float: left; padding: 0em .5em 0em">
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| ==''In silico''==
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| <showhide>
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| {{size|2|'''Sequence Analysis'''}}__HIDER__
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| <hide>
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| • [[BLAST|<font style="color:#000000">BLAST</font>]] <BR>
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| • [[CLUSTALW|<font style="color:#000000">CLUSTALW</font>]] <BR>
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| </hide>
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| </showhide>
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| <showhide>
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| {{size|2|'''Structure'''}}__HIDER__
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| <hide>
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| • [[THREADER|<font style="color:#000000">THREADER</font>]] <BR>
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| • [[Homology Modelling|<font style="color:#000000">Homology Modeling</font>]] <BR>
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| </hide>
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| </showhide>
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| <showhide>
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| {{size|2|'''Pathway Modelling'''}}__HIDER__
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| <hide>
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| • [[FBA|<font style="color:#000000">Flux Balance Analysis</font>]] <BR>
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| • [[MoMA|<font style="color:#000000">Minimisation of Metabolic Adjustment</font>]] <BR>
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| </hide>
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| </showhide>
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| ==Other==
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| <showhide>
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| {{size|2|'''Flow Cytometry'''}}__HIDER__
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| <hide>
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| • [[Flow Calibration|<font style="color:#000000">Flow Calibration</font>]] <BR>
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| </hide>
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| </showhide>
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| <showhide>
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| {{size|2|'''Microfluidics'''}}__HIDER__
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| <hide>
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| • [[Design a Microfluidic Chip|<font style="color:#000000">Design Microfluidic Chip</font>]] <BR>
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| • [[Order a Microfluidic Chip from the Foundry|<font style="color:#000000">Order Microfluidic Chip from Foundry</font>]] <BR>
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| • [[Operate Microfluidic Chemostat|<font style="color:#000000">Operate Microfluidic Chemostat</font>]] <BR>
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| </hide>
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| </showhide>
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| <showhide>
| | Test test test |
| {{size|2|'''Microscopy'''}}__HIDER__
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| <hide>
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| • [[Agarose Pads for Microscopy|<font style="color:#000000">Agarose Pads</font>]] <BR>
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| • [[Quantitative Microscopy|<font style="color:#000000">Quantitative Microscopy</font>]] <BR>
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| • [[Silver: Time-Lapse Microscopy|<font style="color:#000000">Time-Lapse Microscopy</font>]] <BR>
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| </hide>
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| </showhide>
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| <showhide>
| | {|style="text-align:left; background-color:#CDDE95; line-height: 1em" width="25%" cellspacing="0" cellpadding="0" |
| {{size|2|'''Plate reader'''}}__HIDER__ | | <!-----------Active Discussions -----------------> |
| <hide>
| | |{{Click|image=Software.png|width=50px|height=50px|link=Roadmap}} |
| • [[Endy:Victor3 plate reader|<font style="color:#000000">Perkin Elmer Victor<sup>3</sup></font>]]<font size=1>Plate reader usage protocols</font> <BR>
| | |'''[[Roadmap|<font size=2>Development Roadmap</font>]]'''<br /><font size=1>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua.</font> |
| </hide> | | <!----------------------------------------------> |
| </showhide>
| | |}<BR> |
| | | {|style="text-align:left; background-color:#DEE8B8; line-height: 1em" width="25%" cellspacing="0" cellpadding="0" |
| {{size|2|'''General resources'''}}<BR> | | <!-----------Active Discussions -----------------> |
| • [http://protocol-online.org <font style="color:#000000">Protocol-online</font>] <font size=1>Contains a lot of useful protocols and a popular discussion section.</font> <BR>
| | |{{Click|image=Ideas.png|width=50px|height=50px|link=OpenWetWare:Ideas}} |
| | | |'''[[OpenWetWare:Ideas|<font size=2>OWW Ideas</font>]]'''<br /><font size=1>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua.</font> |
| {{size|2|'''Protocols in Development'''}}
| | <!----------------------------------------------> |
| '''Please Contribute!'''<BR> | | |}<BR> |
| • [[Pulse-chase protein production|<font style="color:#000000">Pulse-chase protein production</font>]] <BR>
| | {|style="text-align:left; background-color:#E5EDC8; line-height: 1em" width="25%" cellspacing="0" cellpadding="0" |
| • [[Fixing cells|<font style="color:#000000">Fixing cells</font>]] <BR>
| | <!-----------Active Discussions -----------------> |
| • [[B-galactosidase assay|<font style="color:#000000">β-galactosidase assay</font>]] <BR>
| | |{{Click|image=Design.png|width=50px|height=50px|link=OpenWetWare:Logo and Promo Poster Discussion}} |
| • [[RNA Half-life|<font style="color:#000000">RNA Half-life</font>]] <BR>
| | |'''[[OpenWetWare:Logo and Promo Poster Discussion|<font size=2>Logo and Poster</font>]]'''<br /><font size=1>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua.</font> |
| • [[Denaturing acrylamide gel purification of nucleic acids|<font style="color:#000000">Denaturing acrylamide gel purification of nucleic acids</font>]] <BR>
| | <!----------------------------------------------> |
| • [[Quantification of nucleic acids|<font style="color:#000000">Quantification of nucleic acids</font>]] <BR>
| | |}<BR> |
| </div>
| | {|style="text-align:left; background-color:#C5D0EA; line-height: 1em" width="25%" cellspacing="0" cellpadding="0" |
| | | <!-----------Active Discussions -----------------> |
| |width="170px" style="border: 0px solid #000000; color: #000; background-color: #517AA3" valign="top"|
| | |{{Click|image=InformationManagement.png|width=50px|height=50px|link=Information Management Discussion}} |
| <div style="clear: right; text-align: left; float: left; padding: 0em .5em 0em">
| | |'''[[Information Management Discussion|<font size=2>Info/Design Layouts</font>]]'''<br /><font size=1>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua.</font> |
| ==Header==
| | <!----------------------------------------------> |
| </div>
| | |}<BR> |
| |-
| | {|style="text-align:left; background-color:#B4CE4E; line-height: 1em" width="25%" cellspacing="0" cellpadding="0" |
| | | <!-----------Active Discussions -----------------> |
| |width="188px" style="border: 0px solid #000000; color: #000; background-color: #517AA3" valign="top"|
| | |{{Click|image=CommunityDevelopment.png|width=50px|height=50px|link=Information Management Discussion}} |
| <div style="clear: right; text-align: left; float: left; padding: 0em .5em 0em"> | | |'''[[Information Management Discussion|<font size=2>Info/Design Layouts</font>]]'''<br /><font size=1>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua.</font> |
| ==''In vitro''== | | <!----------------------------------------------> |
| <showhide>
| |
| {{size|2|'''Nucleic acids'''}}__HIDER__
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| <hide>
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| • [[Nucleic acid precipitation|<font style="color:#000000">Nucleic acid precipitation</font>]] <BR>
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| • [[Phenol/chloroform extraction|<font style="color:#000000">Phenol/chloroform extraction</font>]] <BR>
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| </hide> | |
| </showhide>
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| | |
| <showhide>
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| {{size|2|'''DNA'''}}__HIDER__
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| <hide> | |
| • [[Agarose gel electrophoresis|<font style="color:#000000">Agarose gel electrophoresis</font>]] <BR>
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| • [[Annealing Primers|<font style="color:#000000">Annealing Primers</font>]] <BR>
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| • [[Designing primers|<font style="color:#000000">Designing Primers</font>]] <BR>
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| • [[DNA Ligation|<font style="color:#000000">DNA Ligation</font>]] <BR>
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| • [[Library Generation|<font style="color:#000000">Library Generating</font>]] <BR>
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| • [[Miniprep|<font style="color:#000000">Miniprep</font>]] <BR>
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| • [[PCR|<font style="color:#000000">PCR</font>]] <BR>
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| • [[Phosphatase treatment of linearized vector|<font style="color:#000000">Phosphate treatment linearized vector</font>]] <BR>
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| • [http://arep.med.harvard.edu/polony/polony_protocols <font style="color:#000000">Polony Protocols</font>]<font size=1>(Church/Mitra Lab)</font> <BR>
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| • [[PNK Treatment of DNA Ends|<font style="color:#000000">PNK Treatment of DNA ends</font>]] <BR>
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| • [[Purification of DNA|<font style="color:#000000">Purification of DNA</font>]] <BR>
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| • [[Reconstituting primers|<font style="color:#000000">Reconstituting Primers</font>]] <BR>
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| • [[Restriction digest|<font style="color:#000000">Restriction Digest</font>]] <BR>
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| • [['Round-the-horn site-directed mutagenesis|<font style="color:#000000">'Round-the-horn site-directed mutagenesis</font>]] <BR>
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| • [[Sequencing DNA|<font style="color:#000000">Sequencing DNA</font>]] <BR>
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| • [[Site-directed mutagenesis|<font style="color:#000000">Site-directed Mutagenesis</font>]] <BR>
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| • [[Templiphi|<font style="color:#000000">Templiphi</font>]] <BR>
| |
| </hide> | |
| </showhide>
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| | |
| <showhide>
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| {{size|2|'''RNA'''}}__HIDER__
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| <hide> | |
| • [[In vitro transcription with T7 RNA polymerase|<font style="color:#000000">In vitro transcription w/ T7 RNA polymerase</font>]] <BR>
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| • [[Northern Blot, 32P End-Labeled Probes |<font style="color:#000000">Northern Blot, <sup>32</sup>P End-Labeled Probes</font>]] <BR>
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| • [[Probe Prep, 32P End-Labeled Probes|<font style="color:#000000">Probe Prep, <sup>32</sup>P End-Labeled Probes</font>]] <BR>
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| • [[RNA Extraction|<font style="color:#000000">RNA Extraction</font>]] <BR>
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| • [[RNase Protection Assay|<font style="color:#000000">RNAse Protection Assay</font>]] <BR>
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| </hide> | |
| </showhide>
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| | |
| <showhide>
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| {{size|2|'''Protein'''}}__HIDER__ | |
| <hide>
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| • [[Acrylamide Gels|<font style="color:#000000">Acrylamide gels and SDS-PAGE</font>]] <BR>
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| • [[Silver: Destain|<font style="color:#000000">Coomassie Destain</font>]] <BR>
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| • [[Silver: Coomassie Stain|<font style="color:#000000">Coomassie Stain</font>]] <BR>
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| • [[Keating:Experimental Protocols:HPLC|<font style="color:#000000">HPLC</font>]] <BR>
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| • [[Silver staining|<font style="color:#000000">Silver staining</font>]] <BR>
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| • [[Western Blot|<font style="color:#000000">Western Blot</font>]] <BR>
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| • [[Purification of His-tagged proteins|<font style="color:#000000">Purification His-tagged proteins</font>]] <BR>
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| </hide>
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| </showhide>
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| </div>
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| |width="170px" style="border: 0px solid #000000; color: #000; background-color: #6699CC" valign="top"|
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| <div style="clear: right; text-align: left; float: left; padding: 0em .5em 0em">
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| ==Header==
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| </div>
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| |} | | |} |
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| <showhide>
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| __SHOWALL__ __HIDEALL__
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| </showhide>
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| __NOEDITSECTION__
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| __NOTOC__
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