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Protocol News <showhide>__HIDER__<hide></hide></showhide>	In vivo <showhide> Escherichia coli__HIDER__ <hide> • Bacterial cell culture • Bacterial transformation • Preparing chemically competent cells (TSS) • Transforming chemically competent cells (TSS) • Preparing electrocompetent cells • Transforming electrocompetent cells • β-Galactosidase Assay (A better Miller) • Colony PCR • Making a long term stock of bacteria • Miniprep • P1vir phage transduction • Pour LB plates • Protein expression resource • S_N Chemostat Protocol </hide> </showhide> <showhide> Mesoplasma florum__HIDER__ <hide> • Preparing culture media </hide> </showhide> <showhide> T7__HIDER__ <hide> • Cesium Chloride Purification of T7 • Simple lysate protocol from plaque or lysate </hide> </showhide> <showhide> Yeast__HIDER__ <hide> • Assaying mating • Cell Cycle Arrest • Dropout plates for yeast • High Efficiency Transformation • Layered plates • Pour YPD plates • Simple Yeast Lysate (for Western etc.) • Time-Lapse Microscopy • Yeast Colony PCR • Yeast DNA Prep </hide> </showhide>	In silico <showhide> Sequence Analysis__HIDER__ <hide> • BLAST • CLUSTALW </hide> </showhide> <showhide> Structure__HIDER__ <hide> • THREADER • Homology Modelling </hide> </showhide> <showhide> Pathway Modelling__HIDER__ <hide> • Flux Balance Analysis • Minimisation of Metabolic Adjustment </hide> </showhide> Other <showhide> Flow Cytometry__HIDER__ <hide> • Flow Calibration </hide> </showhide> <showhide> Microfluidics__HIDER__ <hide> • Design a Microfluidic Chip • Order a Microfluidic Chip from the Foundry • Operate Microfluidic Chemostat </hide> </showhide> <showhide> Microscopy__HIDER__ <hide> • Agarose Pads for Microscopy • Quantitative Microscopy • Time-Lapse Microscopy </hide> </showhide> <showhide> Plate reader__HIDER__ <hide> • Perkin Elmer Victor3 -Plate reader usage protocols </hide> </showhide> General resources • Protocol-online Contains a lot of useful protocols and a popular discussion section. Protocols in Development Please Contribute! • Pulse-chase protein production • Fixing cells • B-galactosidase assay • RNA Half-life • Denaturing acrylamide gel purification of nucleic acids • Quantification of nucleic acids	Meta • Jobs at OpenWetWare • About OpenWetWare • Steering Committee • Why join? • How to join • Getting Started • Contributing • Other FAQ • Etiquette • Tips • Thoughts on OWW • Science 2.0
	In vitro <showhide> Nucleic acids__HIDER__ <hide> • Nucleic acid precipitation • Phenol/chloroform extraction </hide> </showhide> <showhide> DNA__HIDER__ <hide> • Agarose gel electrophoresis • Annealing Primers • Designing primers • DNA Ligation • Library Generation • Miniprep • PCR • Phosphatase treatment of linearized vector • Polony Protocols (Church/Mitra Lab) • PNK Treatment of DNA Ends • Purification of DNA • Reconstituting primers • Restriction digest • 'Round-the-horn site-directed mutagenesis • Sequencing DNA • Site-directed mutagenesis • Templiphi </hide> </showhide> <showhide> RNA__HIDER__ <hide> • In vitro transcription with T7 RNA polymerase • Northern Blot, 32P End-Labeled Probes • Probe Prep, 32P End-Labeled Probes • RNA Extraction • RNase Protection Assay </hide> </showhide> <showhide> Protein__HIDER__ <hide> • Acrylamide gels and SDS-PAGE • Coomassie Destain • Coomassie Stain • HPLC • Silver staining • Western Blot • Purification of His-tagged proteins </hide> </showhide>		Discussion • Logo and Poster • Information Management • Software Development

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