Modulation of SIR3 Activity and Effects on Aging in Annelids: Difference between revisions
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==Ideas== | ==Ideas== | ||
# | # Knockout SIR3 in Annelids with targeted homologous recombination. | ||
# | # Measure SIR2 Activity | ||
# | # Modulate Translation of SIR3 via RNA interference. Induce plasmids containing complementary sequence to SIR3. | ||
# Controls: In case of knockout, Annelids with intact SIR3,induced with plasmid backbone. In case of RNA interference, induce plasmids containing nonsense sequence. | |||
==Websites== | ==Websites== |
Revision as of 15:23, 27 April 2008
KO of Sir3 in Annelids
Will Gibson and Marta Milan
20.109(S08)
Pink T/R
Begin to define your research proposal by making a wiki page to collect your ideas and resources (you can do this on one page with your partner or split the effort and each turn in an individual page). Keep in mind that your presentation to the class will ultimately need:
- a brief project overview
- sufficient background information for everyone to understand your proposal
- a statement of the research problem and goals
- project details and methods
- predicted outcomes if everything goes according to plan and if nothing does
- needed resources to complete the work
For now, focus on coming up with a research problem and giving a little background about it. Print your user page(s) for next time, making sure it defines your topic, your idea and two or more references you've collected and summarized. Keep in mind that you're not committed to this idea - if you come up with something that you like better later on, that's fine.
Research
- problem
Background
- info
References
- Reference
- Summary
- Reference
- Summary
Ideas
- Knockout SIR3 in Annelids with targeted homologous recombination.
- Measure SIR2 Activity
- Modulate Translation of SIR3 via RNA interference. Induce plasmids containing complementary sequence to SIR3.
- Controls: In case of knockout, Annelids with intact SIR3,induced with plasmid backbone. In case of RNA interference, induce plasmids containing nonsense sequence.
Websites
- <<http://web.mit.edu/newsoffice/2000/yeast-0927.html>>
- <<http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=115252>>
- <<http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=retrieve&dopt=full_report&list_uids=851163>>
- <<http://www.pnas.org/cgi/reprint/97/12/6658?ck=nck>>*
- <<http://en.wikipedia.org/wiki/RNA_interference>>*
- RNA Interference with siRNA's: <<http://en.wikipedia.org/wiki/SiRNA>>*
- Guarante Lab: <<http://web.mit.edu/biology/guarente/about.html>>*
- Knockout Mice: <<http://en.wikipedia.org/wiki/Knockout_mouse>>*