Module 3: our.topic.here: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 2: Line 2:




==Project Overview==
==Brief Project Overview==
Experiments have been performed that effectively showed that RNA interference is a successful method of preventing HIV replication through the regulation of viral genes, or cellular genes necessary for infection.  We would like to explore is in the delivery and localization of the siRNA into undifferentiated embryonic gamete cells for prevention and defense against HIV before an immune response is triggered during the transmission stage of infection. We would specifically like to target helper T cells in the immune response system.
We wish to knock down the viral activities of HIV in mammalian cells by using siRNA to inhibit HIV proliferation.  In order to localize and specify only certain cells, in our case gametes, we wish to incorporate the siRNA into a lentivirus and allowing that lentivirus that do not have any harmful DNA or RNA sequences to attack only certain types of cells.


==Background==
==Background==

Revision as of 02:04, 16 November 2007

A short description of our topic


Brief Project Overview

We wish to knock down the viral activities of HIV in mammalian cells by using siRNA to inhibit HIV proliferation. In order to localize and specify only certain cells, in our case gametes, we wish to incorporate the siRNA into a lentivirus and allowing that lentivirus that do not have any harmful DNA or RNA sequences to attack only certain types of cells.

Background

Applications Any individual with AIDS can receive treatment with RNAi and downregulate AIDS.

Sources

Miguel Angel Martínez, Bonaventura Clotet and José A. Esté. RNA interference of HIV replication http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6W7H-47187D5-1&_user=501045&_coverDate=12%2F01%2F2002&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000022659&_version=1&_urlVersion=0&_userid=501045&md5=d19e8ac4ef0ed43ac644169e32e6c6a0

Martinez, Miguel A a; Gutierrez, Arantxa a; Armand-Ugon, Mercedes a; Blanco, Julia a; Parera, Mariona a; Gomez, Jordi b; Clotet, Bonaventura a; Este, Jose A a. Suppression of chemokine receptor expression by RNA interference allows for inhibition of HIV-1 replication <http://www.aidsonline.com/pt/re/aids/abstract.00002030-200212060-00002.htm;jsessionid=H9FK3pvhMN8x7hw2bG8xF1GsJ19JtFwBB21f4yQJM0GF2TYwTbGz!1600246195!181195629!8091!-1>

Carl D. Novina, Michael F. Murray, Derek M. Dykxhoorn, Paul J. Beresford, Jonathan Riess, Sang-Kyung Lee, Ronald G. Collman, Judy Lieberman, Premlata Shankar & Phillip A. Sharp. siRNA-directed inhibition of HIV-1 infection <http://www.nature.com/nm/journal/v8/n7/abs/nm725.html>

Xiao-Feng Qin, Dong Sung An, Irvin S. Y. Chen, and David Baltimore. Inhibiting HIV-1 infection in human T cells by lentiviral-mediated delivery of small interfering RNA against CCR5 <http://www.pnas.org/cgi/content/abstract/100/1/183>

John Capodici, Katalin Karikó and Drew Weissman. Inhibition of HIV-1 Infection by Small Interfering RNA-Mediated RNA Interference <http://www.jimmunol.org/cgi/content/abstract/169/9/5196>

Research Problem and Goals

Project Details

Please see the Project Details Page

Retrieved from "https://openwetware.org/mediawiki/index.php?title=Module_3:_our.topic.here&oldid=167707"