Moghe:Protocols
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==Cell culture== | ==Cell culture== | ||
| - | * [Moghe:PBMC | PBMC isolation] | + | * [[Moghe:PBMC | PBMC isolation from Buffy Coats]] |
| + | * [[Moghe:MDM| Primary monocyte derived macrophages]] | ||
| + | * [[Moghe:THP-1 | THP-1 Culture/Differentiation]] | ||
| + | * [[Moghe:hMSC | hMSC]] | ||
| + | * [[Moghe:hESC| hESC]] | ||
| - | == | + | ==Analysis== |
| - | ==PCR== | + | ====Microscopy==== |
| + | * [[Moghe:Nikon | Nikon Protocol]] | ||
| + | * [[Moghe:1staining | Staining with conjugated antibodies]] | ||
| + | * [[Moghe:2staining | Staining with secondary antibodies]] | ||
| + | |||
| + | ====PCR==== | ||
| + | * [[Moghe:PCR | qRT-PCR Overview]] | ||
| + | * [[Moghe:primers | Primer Design]] | ||
Current revision
Contents |
Cell culture
- PBMC isolation from Buffy Coats
- Primary monocyte derived macrophages
- THP-1 Culture/Differentiation
- hMSC
- hESC
