Moghe:Protocols: Difference between revisions
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==Cell culture== | ==Cell culture== | ||
* [Moghe:PBMC | PBMC isolation] | * [[Moghe:PBMC | PBMC isolation from Buffy Coats]] | ||
* [[Moghe:MDM| Primary monocyte derived macrophages]] | |||
* [[Moghe:THP-1 | THP-1 Culture/Differentiation]] | |||
* [[Moghe:hMSC | hMSC]] | |||
* [[Moghe:hESC| hESC]] | |||
== | ==Analysis== | ||
==PCR== | ====Microscopy==== | ||
* [[Moghe:Nikon | Nikon Protocol]] | |||
* [[Moghe:1staining | Staining with conjugated antibodies]] | |||
* [[Moghe:2staining | Staining with secondary antibodies]] | |||
====PCR==== | |||
* [[Moghe:PCR | qRT-PCR Overview]] | |||
* [[Moghe:primers | Primer Design]] | |||
Latest revision as of 14:23, 5 October 2010
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Cell culture
- PBMC isolation from Buffy Coats
- Primary monocyte derived macrophages
- THP-1 Culture/Differentiation
- hMSC
- hESC
