Moore:Chemiluminescent: Difference between revisions
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(New page: ==Chemiluminescent HRP Detection for Western Blotting== ===Protocol Background=== ===Recipes of Stocks=== ===Recipes of Working Solutions=== ===Procedure=== ===Comments===) |
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===Protocol Background=== | ===Protocol Background=== | ||
This protocol is presented as a translation from a sheet of paper I received from a colleague. It describes a luminol-based chemiluminescent protocol for detecting horseradish peroxidase-conjugated antibodies during Western immunodetection. This protocol serves to replaces the commercially available ECL and ECL PLus technologies sold and licensed by GE Healthcare. The Protocol I obtained is titled "ECL SOUTHERN / WESTERNS DETECTION REAGENTS" with the footnote "adapted from St. Geme and Miller lab protocols 1/4/01 by her highness Ms. J. Sexton". I don't know who these folks are, but thank you very much, you save us a lot of money. | |||
===Recipes of Stocks=== | ===Recipes of Stocks=== | ||
Revision as of 08:43, 12 November 2008
Chemiluminescent HRP Detection for Western Blotting
Protocol Background
This protocol is presented as a translation from a sheet of paper I received from a colleague. It describes a luminol-based chemiluminescent protocol for detecting horseradish peroxidase-conjugated antibodies during Western immunodetection. This protocol serves to replaces the commercially available ECL and ECL PLus technologies sold and licensed by GE Healthcare. The Protocol I obtained is titled "ECL SOUTHERN / WESTERNS DETECTION REAGENTS" with the footnote "adapted from St. Geme and Miller lab protocols 1/4/01 by her highness Ms. J. Sexton". I don't know who these folks are, but thank you very much, you save us a lot of money.
