Moore:Protocols

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===Cloning and Molecular Biology===
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<br>
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[[Quantitating_nucleic_acids|Figuring Out How Much Nucleic Acid You Have]]<br>
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[[%27Round-the-horn_site-directed_mutagenesis|'Round-the-Horn Mutagenesis]]<br>
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[[Sauer:Stitching_Genes_by_PCR|Gene Stitching]]<br>
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<br>
===Protein Purification===
===Protein Purification===
<br>
<br>
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[[Moore:Poly-U|Poly-U Translation]]<br>
[[Moore:Poly-U|Poly-U Translation]]<br>
[[Moore:mRNA_Translation|mRNA Translation]]<br>
[[Moore:mRNA_Translation|mRNA Translation]]<br>
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<br>
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===RNA Purification and Analysis===
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<br>
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[[Sauer:RNA_Purification_from_E._coli|Getting RNA From ''E. coli'']]<br>

Revision as of 11:39, 2 July 2008

Contents

Cloning and Molecular Biology


Figuring Out How Much Nucleic Acid You Have
'Round-the-Horn Mutagenesis
Gene Stitching

Protein Purification


Getting the Cells open
E. coli ClpX
E. coli ClpP
E. coli SspB

Protein Analysis


Silver Staining
SDS PAGE
Western Blot

Translation and Ribosomes


E. coli Ribosome Purification
Preparing Translation Extracts
Poly-U Translation
mRNA Translation

RNA Purification and Analysis


Getting RNA From E. coli

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