Moore:Protocols

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(E. coli Strain Construction)
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[[Moore:Working_with_coli|Working With ''E. coli'']]<br>
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===Cloning and Molecular Biology===
===Cloning and Molecular Biology===
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Revision as of 12:44, 2 July 2008


Contents

E. coli Strain Construction


Working With E. coli
Transducing Genes with P1 Phage

Cloning and Molecular Biology


Figuring Out How Much Nucleic Acid You Have
'Round-the-Horn Mutagenesis
Gene Stitching

Protein Purification


Getting the Cells open
E. coli ClpX
E. coli ClpP
E. coli SspB

Protein Analysis


Silver Staining
SDS PAGE
Western Blot

Translation and Ribosomes


E. coli Ribosome Purification
Preparing Translation Extracts
Poly-U Translation
mRNA Translation

RNA Purification and Analysis


Getting RNA From E. coli
Primer Extension

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