Moore:Protocols

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Current revision (10:34, 12 November 2008) (view source)
(Protein Analysis)
 
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[[Sauer:ClpX_purification|My cool purifaction]]
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<br>
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===''E. coli'' Strain Construction===
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<br>
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[[Moore:Working_with_coli|Working With ''E. coli'']]<br>
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[[Sauer:P1vir_phage_transduction|Transducing Genes with P1 Phage]]<br>
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<br>
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===Cloning and Molecular Biology===
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<br>
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[[Quantitating_nucleic_acids|Figuring Out How Much Nucleic Acid You Have]]<br>
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[[%27Round-the-horn_site-directed_mutagenesis|'Round-the-Horn Mutagenesis]]<br>
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[[Sauer:Stitching_Genes_by_PCR|Gene Stitching]]<br>
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[[Library_Generation|Making Genomic Libraries]]<br>
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<br>
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===Protein Purification===
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<br>
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[[Sauer:Lysing_E._coli_with_Lysozymes|Getting the Cells open]]<br>
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[[Sauer:ClpX_purification|''E. coli'' ClpX]]<br>
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[[Sauer:ClpP_purification/Untagged_ClpP|''E. coli'' ClpP]]<br>
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[[Sauer:SspB_purification|''E. coli'' SspB]]<br>
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<br>
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===Protein Analysis===
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<br>
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[[Sauer:Silver_staining|Silver Staining]]<br>
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[[Sauer:bis-Tris_SDS-PAGE%2C_the_very_best|SDS PAGE]]<br>
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[[Sauer:Western_blot|Western Blot]]<br>
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[[Moore:Chemiluminescent|Chemiluminescent HRP Detection]]<br>
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===Enymatic Assays===
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[[Beta-Galactosidase_Assay_%28A_better_Miller%29|Miller/β-Galactosidase Assay]]<br>
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===Translation and Ribosomes===
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[[Moore:Ribosome_Purification|''E. coli'' Ribosome Purification]]<br>
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[[Moore:Translation_Extracts|Preparing Translation Extracts]]<br>
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[[Moore:Poly-U|Poly-U Translation]]<br>
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[[Moore:mRNA_Translation|mRNA Translation]]<br>
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===RNA Purification and Analysis===
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[[Sauer:RNA_Purification_from_E._coli|Getting RNA From ''E. coli'']]<br>
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[[Moore:Primer_extension|Primer Extension]]<br>
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<br>

Current revision


Contents

E. coli Strain Construction


Working With E. coli
Transducing Genes with P1 Phage

Cloning and Molecular Biology


Figuring Out How Much Nucleic Acid You Have
'Round-the-Horn Mutagenesis
Gene Stitching
Making Genomic Libraries

Protein Purification


Getting the Cells open
E. coli ClpX
E. coli ClpP
E. coli SspB

Protein Analysis


Silver Staining
SDS PAGE
Western Blot
Chemiluminescent HRP Detection

Enymatic Assays


Miller/β-Galactosidase Assay


Translation and Ribosomes


E. coli Ribosome Purification
Preparing Translation Extracts
Poly-U Translation
mRNA Translation

RNA Purification and Analysis


Getting RNA From E. coli
Primer Extension

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