Moore:Protocols
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| - | [[Sauer:ClpX_purification| | + | <br> |
| + | ===''E. coli'' Strain Construction=== | ||
| + | <br> | ||
| + | [[Moore:Working_with_coli|Working With ''E. coli'']]<br> | ||
| + | [[Sauer:P1vir_phage_transduction|Transducing Genes with P1 Phage]]<br> | ||
| + | <br> | ||
| + | |||
| + | ===Cloning and Molecular Biology=== | ||
| + | <br> | ||
| + | [[Quantitating_nucleic_acids|Figuring Out How Much Nucleic Acid You Have]]<br> | ||
| + | [[%27Round-the-horn_site-directed_mutagenesis|'Round-the-Horn Mutagenesis]]<br> | ||
| + | [[Sauer:Stitching_Genes_by_PCR|Gene Stitching]]<br> | ||
| + | [[Library_Generation|Making Genomic Libraries]]<br> | ||
| + | <br> | ||
| + | ===Protein Purification=== | ||
| + | <br> | ||
| + | [[Sauer:Lysing_E._coli_with_Lysozymes|Getting the Cells open]]<br> | ||
| + | [[Sauer:ClpX_purification|''E. coli'' ClpX]]<br> | ||
| + | [[Sauer:ClpP_purification/Untagged_ClpP|''E. coli'' ClpP]]<br> | ||
| + | [[Sauer:SspB_purification|''E. coli'' SspB]]<br> | ||
| + | <br> | ||
| + | ===Protein Analysis=== | ||
| + | <br> | ||
| + | [[Sauer:Silver_staining|Silver Staining]]<br> | ||
| + | [[Sauer:bis-Tris_SDS-PAGE%2C_the_very_best|SDS PAGE]]<br> | ||
| + | [[Sauer:Western_blot|Western Blot]]<br> | ||
| + | [[Moore:Chemiluminescent|Chemiluminescent HRP Detection]]<br> | ||
| + | <br> | ||
| + | |||
| + | ===Enymatic Assays=== | ||
| + | <br> | ||
| + | [[Beta-Galactosidase_Assay_%28A_better_Miller%29|Miller/β-Galactosidase Assay]]<br> | ||
| + | |||
| + | <br> | ||
| + | ===Translation and Ribosomes=== | ||
| + | <br> | ||
| + | [[Moore:Ribosome_Purification|''E. coli'' Ribosome Purification]]<br> | ||
| + | [[Moore:Translation_Extracts|Preparing Translation Extracts]]<br> | ||
| + | [[Moore:Poly-U|Poly-U Translation]]<br> | ||
| + | [[Moore:mRNA_Translation|mRNA Translation]]<br> | ||
| + | <br> | ||
| + | ===RNA Purification and Analysis=== | ||
| + | <br> | ||
| + | [[Sauer:RNA_Purification_from_E._coli|Getting RNA From ''E. coli'']]<br> | ||
| + | [[Moore:Primer_extension|Primer Extension]]<br> | ||
| + | <br> | ||
Current revision
Contents |
E. coli Strain Construction
Working With E. coli
Transducing Genes with P1 Phage
Cloning and Molecular Biology
Figuring Out How Much Nucleic Acid You Have
'Round-the-Horn Mutagenesis
Gene Stitching
Making Genomic Libraries
Protein Purification
Getting the Cells open
E. coli ClpX
E. coli ClpP
E. coli SspB
Protein Analysis
Silver Staining
SDS PAGE
Western Blot
Chemiluminescent HRP Detection
Enymatic Assays
Translation and Ribosomes
E. coli Ribosome Purification
Preparing Translation Extracts
Poly-U Translation
mRNA Translation
