Moore:Protocols
From OpenWetWare
(Difference between revisions)
(→RNA Purification and Analysis) |
Current revision (11:34, 12 November 2008) (view source) (→Protein Analysis) |
||
| (4 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| + | <br> | ||
| + | ===''E. coli'' Strain Construction=== | ||
| + | <br> | ||
| + | [[Moore:Working_with_coli|Working With ''E. coli'']]<br> | ||
| + | [[Sauer:P1vir_phage_transduction|Transducing Genes with P1 Phage]]<br> | ||
| + | <br> | ||
| + | |||
===Cloning and Molecular Biology=== | ===Cloning and Molecular Biology=== | ||
<br> | <br> | ||
| Line 4: | Line 11: | ||
[[%27Round-the-horn_site-directed_mutagenesis|'Round-the-Horn Mutagenesis]]<br> | [[%27Round-the-horn_site-directed_mutagenesis|'Round-the-Horn Mutagenesis]]<br> | ||
[[Sauer:Stitching_Genes_by_PCR|Gene Stitching]]<br> | [[Sauer:Stitching_Genes_by_PCR|Gene Stitching]]<br> | ||
| + | [[Library_Generation|Making Genomic Libraries]]<br> | ||
<br> | <br> | ||
===Protein Purification=== | ===Protein Purification=== | ||
| Line 17: | Line 25: | ||
[[Sauer:bis-Tris_SDS-PAGE%2C_the_very_best|SDS PAGE]]<br> | [[Sauer:bis-Tris_SDS-PAGE%2C_the_very_best|SDS PAGE]]<br> | ||
[[Sauer:Western_blot|Western Blot]]<br> | [[Sauer:Western_blot|Western Blot]]<br> | ||
| + | [[Moore:Chemiluminescent|Chemiluminescent HRP Detection]]<br> | ||
| + | <br> | ||
| + | |||
| + | ===Enymatic Assays=== | ||
| + | <br> | ||
| + | [[Beta-Galactosidase_Assay_%28A_better_Miller%29|Miller/β-Galactosidase Assay]]<br> | ||
| + | |||
<br> | <br> | ||
===Translation and Ribosomes=== | ===Translation and Ribosomes=== | ||
Current revision
Contents |
E. coli Strain Construction
Working With E. coli
Transducing Genes with P1 Phage
Cloning and Molecular Biology
Figuring Out How Much Nucleic Acid You Have
'Round-the-Horn Mutagenesis
Gene Stitching
Making Genomic Libraries
Protein Purification
Getting the Cells open
E. coli ClpX
E. coli ClpP
E. coli SspB
Protein Analysis
Silver Staining
SDS PAGE
Western Blot
Chemiluminescent HRP Detection
Enymatic Assays
Translation and Ribosomes
E. coli Ribosome Purification
Preparing Translation Extracts
Poly-U Translation
mRNA Translation
