Moore:Protocols: Difference between revisions
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[[Sauer:Stitching_Genes_by_PCR|Gene Stitching]]<br> | [[Sauer:Stitching_Genes_by_PCR|Gene Stitching]]<br> | ||
[[Library_Generation|Making Genomic Libraries]]<br> | [[Library_Generation|Making Genomic Libraries]]<br> | ||
[[ | [[Extracting_Genomic_DNA_from_E. coli|Genomic DNA Prep]] <br> | ||
<br> | <br> | ||
Revision as of 17:54, 7 June 2016
E. coli Strain Construction
Working With E. coli
Transducing Genes with P1 Phage
Cloning and Molecular Biology
Figuring Out How Much Nucleic Acid You Have
'Round-the-Horn Mutagenesis
Gene Stitching
Making Genomic Libraries
Genomic DNA Prep
Protein Purification
Getting the Cells open
E. coli ClpX
E. coli ClpP
E. coli SspB
Protein Analysis
Silver Staining
SDS PAGE
Western Blot
Chemiluminescent HRP Detection
Enymatic Assays
Translation and Ribosomes
E. coli Ribosome Purification
Preparing Translation Extracts
Poly-U Translation
mRNA Translation
