Moore:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
| (12 intermediate revisions by 2 users not shown) | |||
| Line 1: | Line 1: | ||
<br> | |||
===''E. coli'' Strain Construction=== | |||
<br> | |||
[[Moore:Working_with_coli|Working With ''E. coli'']]<br> | |||
[[Sauer:P1vir_phage_transduction|Transducing Genes with P1 Phage]]<br> | |||
<br> | |||
===Cloning and Molecular Biology=== | |||
<br> | |||
[[Moore Lab TBE |Moore Lab TBE Recipe]]<br> | |||
[[Quantitating_nucleic_acids|Figuring Out How Much Nucleic Acid You Have]]<br> | |||
[[%27Round-the-horn_site-directed_mutagenesis|'Round-the-Horn Mutagenesis]]<br> | |||
[[Sauer:Stitching_Genes_by_PCR|Gene Stitching]]<br> | |||
[[Extracting_Genomic_DNA_from_E. coli|Genomic DNA Prep]] <br> | |||
[[Library_Generation|Making Genomic Libraries]]<br> | |||
<br> | |||
===Protein Purification=== | ===Protein Purification=== | ||
<br> | <br> | ||
| Line 11: | Line 28: | ||
[[Sauer:bis-Tris_SDS-PAGE%2C_the_very_best|SDS PAGE]]<br> | [[Sauer:bis-Tris_SDS-PAGE%2C_the_very_best|SDS PAGE]]<br> | ||
[[Sauer:Western_blot|Western Blot]]<br> | [[Sauer:Western_blot|Western Blot]]<br> | ||
[[Moore:Chemiluminescent|Chemiluminescent HRP Detection]]<br> | |||
<br> | |||
===Enymatic Assays=== | |||
<br> | |||
[[Beta-Galactosidase_Assay_%28A_better_Miller%29|Miller/β-Galactosidase Assay]]<br> | |||
<br> | <br> | ||
===Translation and Ribosomes=== | ===Translation and Ribosomes=== | ||
| Line 18: | Line 42: | ||
[[Moore:Poly-U|Poly-U Translation]]<br> | [[Moore:Poly-U|Poly-U Translation]]<br> | ||
[[Moore:mRNA_Translation|mRNA Translation]]<br> | [[Moore:mRNA_Translation|mRNA Translation]]<br> | ||
[[Rate Zonal Centrifugation|Harvesting Polysomes]]<br> | |||
<br> | |||
===RNA Purification and Analysis=== | |||
<br> | |||
[[Sauer:RNA_Purification_from_E._coli|Getting RNA From ''E. coli'']]<br> | |||
[[Moore:RNA_Gels|RNA Gels]]<br> | |||
[[Moore:Primer_extension|Primer Extension]]<br> | |||
<br> | |||
Latest revision as of 13:43, 5 March 2020
E. coli Strain Construction
Working With E. coli
Transducing Genes with P1 Phage
Cloning and Molecular Biology
Moore Lab TBE Recipe
Figuring Out How Much Nucleic Acid You Have
'Round-the-Horn Mutagenesis
Gene Stitching
Genomic DNA Prep
Making Genomic Libraries
Protein Purification
Getting the Cells open
E. coli ClpX
E. coli ClpP
E. coli SspB
Protein Analysis
Silver Staining
SDS PAGE
Western Blot
Chemiluminescent HRP Detection
Enymatic Assays
Translation and Ribosomes
E. coli Ribosome Purification
Preparing Translation Extracts
Poly-U Translation
mRNA Translation
Harvesting Polysomes
