Moore:Protocols: Difference between revisions
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===Cloning and Molecular Biology=== | ===Cloning and Molecular Biology=== | ||
<br> | <br> | ||
[[Moore Lab TBE |Moore Lab TBE Recipe]]<br> | |||
[[Quantitating_nucleic_acids|Figuring Out How Much Nucleic Acid You Have]]<br> | [[Quantitating_nucleic_acids|Figuring Out How Much Nucleic Acid You Have]]<br> | ||
[[%27Round-the-horn_site-directed_mutagenesis|'Round-the-Horn Mutagenesis]]<br> | [[%27Round-the-horn_site-directed_mutagenesis|'Round-the-Horn Mutagenesis]]<br> | ||
[[Sauer:Stitching_Genes_by_PCR|Gene Stitching]]<br> | [[Sauer:Stitching_Genes_by_PCR|Gene Stitching]]<br> | ||
[[Library_Generation|Making Genomic Libraries]]<br> | [[Extracting_Genomic_DNA_from_E. coli|Genomic DNA Prep]] <br> | ||
[[Library_Generation|Making Genomic Libraries]]<br> | |||
<br> | <br> | ||
===Protein Purification=== | ===Protein Purification=== | ||
<br> | <br> | ||
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[[Sauer:bis-Tris_SDS-PAGE%2C_the_very_best|SDS PAGE]]<br> | [[Sauer:bis-Tris_SDS-PAGE%2C_the_very_best|SDS PAGE]]<br> | ||
[[Sauer:Western_blot|Western Blot]]<br> | [[Sauer:Western_blot|Western Blot]]<br> | ||
[[Moore:Chemiluminescent|Chemiluminescent HRP Detection]]<br> | |||
<br> | <br> | ||
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[[Moore:Poly-U|Poly-U Translation]]<br> | [[Moore:Poly-U|Poly-U Translation]]<br> | ||
[[Moore:mRNA_Translation|mRNA Translation]]<br> | [[Moore:mRNA_Translation|mRNA Translation]]<br> | ||
[[Rate Zonal Centrifugation|Harvesting Polysomes]]<br> | |||
<br> | <br> | ||
===RNA Purification and Analysis=== | ===RNA Purification and Analysis=== | ||
<br> | <br> | ||
[[Sauer:RNA_Purification_from_E._coli|Getting RNA From ''E. coli'']]<br> | [[Sauer:RNA_Purification_from_E._coli|Getting RNA From ''E. coli'']]<br> | ||
[[Moore:RNA_Gels|RNA Gels]]<br> | |||
[[Moore:Primer_extension|Primer Extension]]<br> | [[Moore:Primer_extension|Primer Extension]]<br> | ||
<br> | <br> | ||
Latest revision as of 13:43, 5 March 2020
E. coli Strain Construction
Working With E. coli
Transducing Genes with P1 Phage
Cloning and Molecular Biology
Moore Lab TBE Recipe
Figuring Out How Much Nucleic Acid You Have
'Round-the-Horn Mutagenesis
Gene Stitching
Genomic DNA Prep
Making Genomic Libraries
Protein Purification
Getting the Cells open
E. coli ClpX
E. coli ClpP
E. coli SspB
Protein Analysis
Silver Staining
SDS PAGE
Western Blot
Chemiluminescent HRP Detection
Enymatic Assays
Translation and Ribosomes
E. coli Ribosome Purification
Preparing Translation Extracts
Poly-U Translation
mRNA Translation
Harvesting Polysomes
