Moore:Protocols - Revision history

Smoore: /* Protein Analysis */

Smoore — Wed, 12 Nov 2008 15:34:33 GMT

Protein Analysis

←Older revision		Revision as of 15:34, 12 November 2008
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	[[Sauer:bis-Tris_SDS-PAGE%2C_the_very_best\|SDS PAGE]]<br>		[[Sauer:bis-Tris_SDS-PAGE%2C_the_very_best\|SDS PAGE]]<br>
	[[Sauer:Western_blot\|Western Blot]]<br>		[[Sauer:Western_blot\|Western Blot]]<br>
		+	[[Moore:Chemiluminescent\|Chemiluminescent HRP Detection]]<br>
	<br>		<br>

Smoore — Wed, 02 Jul 2008 21:55:59 GMT

←Older revision		Revision as of 21:55, 2 July 2008
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	[[%27Round-the-horn_site-directed_mutagenesis\|'Round-the-Horn Mutagenesis]]<br>		[[%27Round-the-horn_site-directed_mutagenesis\|'Round-the-Horn Mutagenesis]]<br>
	[[Sauer:Stitching_Genes_by_PCR\|Gene Stitching]]<br>		[[Sauer:Stitching_Genes_by_PCR\|Gene Stitching]]<br>
		+	[[Library_Generation\|Making Genomic Libraries]]<br>
	<br>		<br>
	===Protein Purification===		===Protein Purification===
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	[[Sauer:bis-Tris_SDS-PAGE%2C_the_very_best\|SDS PAGE]]<br>		[[Sauer:bis-Tris_SDS-PAGE%2C_the_very_best\|SDS PAGE]]<br>
	[[Sauer:Western_blot\|Western Blot]]<br>		[[Sauer:Western_blot\|Western Blot]]<br>
		+	<br>
		+
		+	===Enymatic Assays===
		+	<br>
		+	[[Beta-Galactosidase_Assay_%28A_better_Miller%29\|Miller/β-Galactosidase Assay]]<br>
		+
	<br>		<br>
	===Translation and Ribosomes===		===Translation and Ribosomes===

Smoore — Wed, 02 Jul 2008 16:44:20 GMT

E. coli Strain Construction

←Older revision		Revision as of 16:44, 2 July 2008
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	<br>		<br>
	[[Moore:Working_with_coli\|Working With ''E. coli'']]<br>		[[Moore:Working_with_coli\|Working With ''E. coli'']]<br>
-	[[Sauer:P1vir_phage_transduction\|Transducing Genes with P1 Phage}}<br>	+	[[Sauer:P1vir_phage_transduction\|Transducing Genes with P1 Phage]]<br>
	<br>		<br>
		+
	===Cloning and Molecular Biology===		===Cloning and Molecular Biology===
	<br>		<br>

Smoore — Wed, 02 Jul 2008 16:44:01 GMT

Smoore — Wed, 02 Jul 2008 16:43:46 GMT

←Older revision		Revision as of 16:43, 2 July 2008
Line 1:		Line 1:
		+	===''E. coli'' Strain Construction===
		+	<br>
		+	[[Moore:Working_with_coli\|Working With ''E. coli'']]<br>
		+	[[Sauer:P1vir_phage_transduction\|Transducing Genes with P1 Phage}}<br>
		+	<br>
	===Cloning and Molecular Biology===		===Cloning and Molecular Biology===
	<br>		<br>

Smoore — Wed, 02 Jul 2008 16:40:35 GMT

RNA Purification and Analysis

←Older revision		Revision as of 16:40, 2 July 2008
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	<br>		<br>
	[[Sauer:RNA_Purification_from_E._coli\|Getting RNA From ''E. coli'']]<br>		[[Sauer:RNA_Purification_from_E._coli\|Getting RNA From ''E. coli'']]<br>
		+	[[Moore:Primer_extension\|Primer Extension]]<br>
		+	<br>

Smoore — Wed, 02 Jul 2008 16:39:32 GMT

←Older revision		Revision as of 16:39, 2 July 2008
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		+	===Cloning and Molecular Biology===
		+	<br>
		+	[[Quantitating_nucleic_acids\|Figuring Out How Much Nucleic Acid You Have]]<br>
		+	[[%27Round-the-horn_site-directed_mutagenesis\|'Round-the-Horn Mutagenesis]]<br>
		+	[[Sauer:Stitching_Genes_by_PCR\|Gene Stitching]]<br>
		+	<br>
	===Protein Purification===		===Protein Purification===
	<br>		<br>
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	[[Moore:Poly-U\|Poly-U Translation]]<br>		[[Moore:Poly-U\|Poly-U Translation]]<br>
	[[Moore:mRNA_Translation\|mRNA Translation]]<br>		[[Moore:mRNA_Translation\|mRNA Translation]]<br>
		+	<br>
		+	===RNA Purification and Analysis===
		+	<br>
		+	[[Sauer:RNA_Purification_from_E._coli\|Getting RNA From ''E. coli'']]<br>

Smoore — Wed, 02 Jul 2008 16:29:36 GMT

←Older revision		Revision as of 16:29, 2 July 2008
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	[[Sauer:Western_blot\|Western Blot]]<br>		[[Sauer:Western_blot\|Western Blot]]<br>
	<br>		<br>
		+	===Translation and Ribosomes===
		+	<br>
		+	[[Moore:Ribosome_Purification\|''E. coli'' Ribosome Purification]]<br>
		+	[[Moore:Translation_Extracts\|Preparing Translation Extracts]]<br>
		+	[[Moore:Poly-U\|Poly-U Translation]]<br>
		+	[[Moore:mRNA_Translation\|mRNA Translation]]<br>

Smoore — Wed, 02 Jul 2008 16:22:45 GMT

←Older revision		Revision as of 16:22, 2 July 2008
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-	===Protein Purification===<br>	+	===Protein Purification===
		+	<br>
	[[Sauer:Lysing_E._coli_with_Lysozymes\|Getting the Cells open]]<br>		[[Sauer:Lysing_E._coli_with_Lysozymes\|Getting the Cells open]]<br>
	[[Sauer:ClpX_purification\|''E. coli'' ClpX]]<br>		[[Sauer:ClpX_purification\|''E. coli'' ClpX]]<br>
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	[[Sauer:SspB_purification\|''E. coli'' SspB]]<br>		[[Sauer:SspB_purification\|''E. coli'' SspB]]<br>
	<br>		<br>
-	===Protein Analysis===<br>	+	===Protein Analysis===
		+	<br>
	[[Sauer:Silver_staining\|Silver Staining]]<br>		[[Sauer:Silver_staining\|Silver Staining]]<br>
	[[Sauer:bis-Tris_SDS-PAGE%2C_the_very_best\|SDS PAGE]]<br>		[[Sauer:bis-Tris_SDS-PAGE%2C_the_very_best\|SDS PAGE]]<br>
	[[Sauer:Western_blot\|Western Blot]]<br>		[[Sauer:Western_blot\|Western Blot]]<br>
	<br>		<br>

Smoore — Wed, 02 Jul 2008 16:21:46 GMT

←Older revision		Revision as of 16:21, 2 July 2008
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-	==Protein Purification==<br>	+	===Protein Purification===<br>
	[[Sauer:Lysing_E._coli_with_Lysozymes\|Getting the Cells open]]<br>		[[Sauer:Lysing_E._coli_with_Lysozymes\|Getting the Cells open]]<br>
	[[Sauer:ClpX_purification\|''E. coli'' ClpX]]<br>		[[Sauer:ClpX_purification\|''E. coli'' ClpX]]<br>
Line 5:		Line 5:
	[[Sauer:SspB_purification\|''E. coli'' SspB]]<br>		[[Sauer:SspB_purification\|''E. coli'' SspB]]<br>
	<br>		<br>
-	==Protein Analysis==<br>	+	===Protein Analysis===<br>
	[[Sauer:Silver_staining\|Silver Staining]]<br>		[[Sauer:Silver_staining\|Silver Staining]]<br>
	[[Sauer:bis-Tris_SDS-PAGE%2C_the_very_best\|SDS PAGE]]<br>		[[Sauer:bis-Tris_SDS-PAGE%2C_the_very_best\|SDS PAGE]]<br>
	[[Sauer:Western_blot\|Western Blot]]<br>		[[Sauer:Western_blot\|Western Blot]]<br>
	<br>		<br>