Muratore:Materials/Media/LB: Difference between revisions
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*1 g NaCl | *1 g NaCl | ||
→ add water to 50 mL (use graduated cylinder) and transfer to 200 mL Wheaton bottles | → add water to 50 mL (use graduated cylinder) and transfer to 200 mL Wheaton bottles | ||
→ autoclave with caps loose | → autoclave with caps loose | ||
→ store long term at 4°C or for immediate use at room T | → store long term at 4°C or for immediate use at room T | ||
[[Category:Material]] | [[Category:Material]] | ||
[[Category:Media]] | [[Category:Media]] | ||
Revision as of 11:09, 26 May 2011
Bottles of 50 mL LB:
- 1 g Bacto-tryptone
- 0.5 g Yeast extract
- 1 g NaCl
→ add water to 50 mL (use graduated cylinder) and transfer to 200 mL Wheaton bottles
→ autoclave with caps loose
→ store long term at 4°C or for immediate use at room T
