Muratore:Protocols/Digest/NheI: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
m (Muratore:Protocol/Digest/NheI moved to Muratore:Protocols/Digest/NheI: Consistent naming) |
||
| (2 intermediate revisions by the same user not shown) | |||
| Line 11: | Line 11: | ||
|24.5 μL ||5 μL ||0.5 μL ||15 μL from purified PCR reaction ||5μL | |24.5 μL ||5 μL ||0.5 μL ||15 μL from purified PCR reaction ||5μL | ||
|} | |} | ||
#2h @ 37°C | |||
#20' @ 65°C (heat kill) | |||
[[Category:Protocol]] | |||
[[Category:DNA]] | |||
Latest revision as of 14:53, 26 May 2011
| Tube | sterile H2O | 10X NEB2 | 100X BSA | DNA | NheI (10 kU/mL) |
|---|---|---|---|---|---|
| plasmid | 34.5 μL | 5 μL | 0.5 μL | 5 μL of 100-500 μg/mL | 5μL |
| insert | 24.5 μL | 5 μL | 0.5 μL | 15 μL from purified PCR reaction | 5μL |
- 2h @ 37°C
- 20' @ 65°C (heat kill)
