Muratore:Protocols/Digest/NheI: Difference between revisions
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(New page: {|border="1" |+ '''NheI digest protocol''' |- !Tube !!sterile H<sub>2</sub>O !!10X NEB2 !!100X BSA !!DNA !!NheI (10 kU/mL) |- !plasmid |34.5 μL ||5 μL ||0.5 μL ||5 μL of 100-500 μg/mL...) |
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|24.5 μL ||5 μL ||0.5 μL ||15 μL from purified PCR reaction ||5μL | |24.5 μL ||5 μL ||0.5 μL ||15 μL from purified PCR reaction ||5μL | ||
|} | |} | ||
;&rarr# 2h @ 37°C | |||
# 20' @ 65°C (heat kill) |
Revision as of 10:55, 26 May 2011
Tube | sterile H2O | 10X NEB2 | 100X BSA | DNA | NheI (10 kU/mL) |
---|---|---|---|---|---|
plasmid | 34.5 μL | 5 μL | 0.5 μL | 5 μL of 100-500 μg/mL | 5μL |
insert | 24.5 μL | 5 μL | 0.5 μL | 15 μL from purified PCR reaction | 5μL |
- &rarr# 2h @ 37°C
- 20' @ 65°C (heat kill)