Muratore:Protocols/Digest/NheI: Difference between revisions
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|24.5 μL ||5 μL ||0.5 μL ||15 μL from purified PCR reaction ||5μL | |24.5 μL ||5 μL ||0.5 μL ||15 μL from purified PCR reaction ||5μL | ||
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→# 2h @ 37°C | → | ||
:# 2h @ 37°C | |||
:# 20' @ 65°C (heat kill) | :# 20' @ 65°C (heat kill) | ||
Revision as of 10:56, 26 May 2011
| Tube | sterile H2O | 10X NEB2 | 100X BSA | DNA | NheI (10 kU/mL) |
|---|---|---|---|---|---|
| plasmid | 34.5 μL | 5 μL | 0.5 μL | 5 μL of 100-500 μg/mL | 5μL |
| insert | 24.5 μL | 5 μL | 0.5 μL | 15 μL from purified PCR reaction | 5μL |
→
- 2h @ 37°C
- 20' @ 65°C (heat kill)
