Muratore:Protocols/Digest/NheI: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
mNo edit summary |
m (adding categories) |
||
| Line 13: | Line 13: | ||
#2h @ 37°C | #2h @ 37°C | ||
#20' @ 65°C (heat kill) | #20' @ 65°C (heat kill) | ||
[[Category:Protocol]] | |||
[[Category:DNA]] | |||
Revision as of 10:58, 26 May 2011
| Tube | sterile H2O | 10X NEB2 | 100X BSA | DNA | NheI (10 kU/mL) |
|---|---|---|---|---|---|
| plasmid | 34.5 μL | 5 μL | 0.5 μL | 5 μL of 100-500 μg/mL | 5μL |
| insert | 24.5 μL | 5 μL | 0.5 μL | 15 μL from purified PCR reaction | 5μL |
- 2h @ 37°C
- 20' @ 65°C (heat kill)
