http://www.openwetware.org/index.php?title=Muratore:Protocols/PCR/Basic&feed=atom&action=history 2013-05-18T13:43:28Z Revision history for this page on the wiki MediaWiki 1.13.2 http://www.openwetware.org/index.php?title=Muratore:Protocols/PCR/Basic&diff=514952&oldid=prev 2011-06-08T21:06:21Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 21:06, 8 June 2011</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 3:</td> <td colspan="2" class="diff-lineno">Line 3:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Basic PCR protocol'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Basic PCR protocol'''</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>!Tube !!sterile H&lt;sub&gt;2&lt;/sub&gt;O !!Pfu Buffer !!Template !!5'primer !!3'primer !!dNTPs !!Pfu Turbo !!wax</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>!Tube !!sterile H&lt;sub&gt;2&lt;/sub&gt;O !!Pfu Buffer !!Template !!5'primer !!3'primer !!<ins class="diffchange diffchange-inline">[[</ins>dNTPs<ins class="diffchange diffchange-inline">]] </ins>!!<ins class="diffchange diffchange-inline">[[</ins>Pfu Turbo<ins class="diffchange diffchange-inline">]] </ins>!!wax</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>! !! !!(10X) !!(10 μg/mL) !!(4 μM) !!(4 μM) !!(10 mM ea) !!(2.5 U/μL) !!</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>! !! !!(10X) !!(10 μg/mL) !!(4 μM) !!(4 μM) !!(10 mM ea) !!(2.5 U/μL) !!</div></td></tr> <!-- diff generator: internal 2013-05-18 13:43:28 --> </table>

Kathryn Muratore http://www.openwetware.org/index.php?title=Muratore:Protocols/PCR/Basic&diff=514951&oldid=prev 2011-06-08T21:05:27Z

<p>Undo revision 514657 by <a href="/wiki/Special:Contributions/Kathryn_Muratore" title="Special:Contributions/Kathryn Muratore">Kathryn Muratore</a> (<a href="/wiki/User_talk:Kathryn_Muratore" title="User talk:Kathryn Muratore">Talk</a>)</p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 21:05, 8 June 2011</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">==Basic PCR protocol==</del></div></td><td colspan="2">&nbsp;</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|border=&quot;1&quot;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|border=&quot;1&quot;</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+</div></td></tr> <!-- diff generator: internal 2013-05-18 13:43:28 --> </table>

Kathryn Muratore http://www.openwetware.org/index.php?title=Muratore:Protocols/PCR/Basic&diff=514949&oldid=prev 2011-06-08T21:05:02Z

<p><a href="/wiki/Muratore:Protocols/PCR" title="Muratore:Protocols/PCR">Muratore:Protocols/PCR</a> moved to <a href="/wiki/Muratore:Protocols/PCR/Basic" title="Muratore:Protocols/PCR/Basic">Muratore:Protocols/PCR/Basic</a> over redirect</p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 21:05, 8 June 2011</td> </tr> <!-- diff generator: internal 2013-05-18 13:43:28 --> </table>

Kathryn Muratore http://www.openwetware.org/index.php?title=Muratore:Protocols/PCR/Basic&diff=514657&oldid=prev 2011-06-08T14:25:43Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 14:25, 8 June 2011</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==Basic PCR protocol==</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|border=&quot;1&quot;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|border=&quot;1&quot;</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+</div></td></tr> <!-- diff generator: internal 2013-05-18 13:43:28 --> </table>

Kathryn Muratore http://www.openwetware.org/index.php?title=Muratore:Protocols/PCR/Basic&diff=514653&oldid=prev 2011-06-08T14:23:56Z

<p><a href="/wiki/Muratore:Protocols/PCR/Basic" title="Muratore:Protocols/PCR/Basic">Muratore:Protocols/PCR/Basic</a> moved to <a href="/wiki/Muratore:Protocols/PCR" title="Muratore:Protocols/PCR">Muratore:Protocols/PCR</a></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 14:23, 8 June 2011</td> </tr> <!-- diff generator: internal 2013-05-18 13:43:28 --> </table>

Kathryn Muratore http://www.openwetware.org/index.php?title=Muratore:Protocols/PCR/Basic&diff=511454&oldid=prev 2011-05-26T17:45:21Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 17:45, 26 May 2011</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 22:</td> <td colspan="2" class="diff-lineno">Line 22:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:Protocol]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:Protocol]]</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Category:DNA]]</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:PCR]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:PCR]]</div></td></tr> <!-- diff generator: internal 2013-05-18 13:43:28 --> </table>

Kathryn Muratore http://www.openwetware.org/index.php?title=Muratore:Protocols/PCR/Basic&diff=511453&oldid=prev 2011-05-26T17:45:04Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 17:45, 26 May 2011</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 20:</td> <td colspan="2" class="diff-lineno">Line 20:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># repeat steps 2-4 x 29 more times</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># repeat steps 2-4 x 29 more times</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># 5' @ 78°C</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># 5' @ 78°C</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Category:Protocol]]</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Category:PCR]]</ins></div></td></tr> <!-- diff generator: internal 2013-05-18 13:43:28 --> </table>

Kathryn Muratore http://www.openwetware.org/index.php?title=Muratore:Protocols/PCR/Basic&diff=511450&oldid=prev 2011-05-26T17:42:05Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 17:42, 26 May 2011</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|border=&quot;1&quot;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|border=&quot;1&quot;</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Basic PCR protocol</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">'''</ins>Basic PCR protocol<ins class="diffchange diffchange-inline">'''</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>!Tube !!sterile H&lt;sub&gt;2&lt;/sub&gt;O !!Pfu Buffer !!Template !!5'primer !!3'primer !!dNTPs !!Pfu Turbo !!wax</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>!Tube !!sterile H&lt;sub&gt;2&lt;/sub&gt;O !!Pfu Buffer !!Template !!5'primer !!3'primer !!dNTPs !!Pfu Turbo !!wax</div></td></tr> <tr><td colspan="2" class="diff-lineno">Line 7:</td> <td colspan="2" class="diff-lineno">Line 7:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>! !! !!(10X) !!(10 μg/mL) !!(4 μM) !!(4 μM) !!(10 mM ea) !!(2.5 U/μL) !!</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>! !! !!(10X) !!(10 μg/mL) !!(4 μM) !!(4 μM) !!(10 mM ea) !!(2.5 U/μL) !!</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>!<del class="diffchange diffchange-inline">A</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>!<ins class="diffchange diffchange-inline">Experimental</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|72 μL ||10 μL ||5 μL ||5 μL ||5 μL ||2 μL ||1 μL ||50 μL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|72 μL ||10 μL ||5 μL ||5 μL ||5 μL ||2 μL ||1 μL ||50 μL</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>!<del class="diffchange diffchange-inline">B</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>!<ins class="diffchange diffchange-inline">(-) Control</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|73 μL ||10 μL ||5 μL ||5 μL ||5 μL ||2 μL ||0 μL ||50 μL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|73 μL ||10 μL ||5 μL ||5 μL ||5 μL ||2 μL ||0 μL ||50 μL</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td></tr> <!-- diff generator: internal 2013-05-18 13:43:28 --> </table>

Kathryn Muratore http://www.openwetware.org/index.php?title=Muratore:Protocols/PCR/Basic&diff=511448&oldid=prev 2011-05-26T17:41:20Z

<p>New page: {|border=&quot;1&quot; |+ Basic PCR protocol |- !Tube !!sterile H&lt;sub&gt;2&lt;/sub&gt;O !!Pfu Buffer !!Template !!5'primer !!3'primer !!dNTPs !!Pfu Turbo !!wax |- ! !! !!(10X) !!(10 μg/mL) !!(4 μM) !!(4 μ...</p> <p><b>New page</b></p><div>{|border=&quot;1&quot;<br /> |+<br /> Basic PCR protocol<br /> |-<br /> !Tube !!sterile H&lt;sub&gt;2&lt;/sub&gt;O !!Pfu Buffer !!Template !!5'primer !!3'primer !!dNTPs !!Pfu Turbo !!wax<br /> |-<br /> ! !! !!(10X) !!(10 μg/mL) !!(4 μM) !!(4 μM) !!(10 mM ea) !!(2.5 U/μL) !!<br /> |-<br /> !A<br /> |72 μL ||10 μL ||5 μL ||5 μL ||5 μL ||2 μL ||1 μL ||50 μL<br /> |-<br /> !B<br /> |73 μL ||10 μL ||5 μL ||5 μL ||5 μL ||2 μL ||0 μL ||50 μL<br /> |}<br /> * Add each from left to right. Mix and spin before adding Pfu Turbo. Gentle mixing and spin after Pfu Turbo. Don't mix in wax.<br /> # 5' @ 95°C<br /> # 30&quot; @ 95°C<br /> # 2' @ T&lt;sub&gt;m&lt;/sub&gt;-5°C<br /> # 2' @ 78°C<br /> # repeat steps 2-4 x 29 more times<br /> # 5' @ 78°C</div>

Kathryn Muratore