http://www.openwetware.org/index.php?title=Muratore:Protocols/Transformation&action=history Revision history for this page on the wiki en MediaWiki 1.13.2 Sat, 25 May 2013 10:18:09 GMT http://www.openwetware.org/index.php?title=Muratore:Protocols/Transformation&diff=515020&oldid=prev <p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 01:11, 9 June 2011</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 11:</td> <td colspan="2" class="diff-lineno">Line 11:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>##* spread remaining 800 μL on fresh plates if poor/no growth observed</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>##* spread remaining 800 μL on fresh plates if poor/no growth observed</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>##* otherwise, discard remainder after ~24h</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>##* otherwise, discard remainder after ~24h</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Category:Protocol]]</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Category:DNA]]</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Category:E. coli]]</ins></div></td></tr> <!-- diff generator: internal 2013-05-25 10:18:09 --> </table> Thu, 09 Jun 2011 01:11:53 GMT Kathryn Muratore http://www.openwetware.org/wiki/Talk:Muratore:Protocols/Transformation http://www.openwetware.org/index.php?title=Muratore:Protocols/Transformation&diff=515019&oldid=prev <p>New page: ==Bacterial Transformation== # 200 μL chemically-<a href="/index.php?title=Muratore:Protocols/Competent_cells&amp;action=edit&amp;redlink=1" class="new" title="Muratore:Protocols/Competent cells (not yet written)"> competent</a> ''E. coli'' + 5 μL <a href="/wiki/DNA" title="DNA">DNA</a> # 30 min on ice # 30 sec @ 42°C # 2 min on ice # add 800 μ...</p> <p><b>New page</b></p><div>==Bacterial Transformation==<br /> # 200 μL chemically-[[Muratore:Protocols/Competent cells| competent]] ''E. coli'' + 5 μL [[DNA]]<br /> # 30 min on ice<br /> # 30 sec @ 42°C<br /> # 2 min on ice<br /> # add 800 μL [[Muratore:Materials/Media/SOC| SOC]]<br /> # 45 min @ 37°C<br /> ## spread 200 μL on rich-[[media]] plates<br /> ##&amp;rarr; 37°C O/N (inverted)<br /> ## store remainder @ 4°C<br /> ##* spread remaining 800 μL on fresh plates if poor/no growth observed<br /> ##* otherwise, discard remainder after ~24h</div> Thu, 09 Jun 2011 01:09:49 GMT Kathryn Muratore http://www.openwetware.org/wiki/Talk:Muratore:Protocols/Transformation