NDF

Overview

Replace this sentence with a brief description of the protocol and its goal.

Materials

• sodium sulfite (stored in chemical cabinets)
• top-loading balance (2 decimal places)
• spatula
• alpha-amylase (stored in refrigerator)
• 5 mL pipet
• neutral detergent solution (stored in room 113B)
• 2000 mL flask
• acetone (in flammables cabinet below fume hood in room 113B)
• digestion apparatus (ANKOM fiber analyzer)
• analytical balance
• drying oven at 105oC
• dessicator
• zip-loc bags with dessicant pouches (recently recharged at 105oC)
• 3 glass jars
• microwave
• hazardous waste container

Procedure

1. Step 1
2. Step 2
   • Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
3. Step 3
   1. Step 3 has multiple sub-steps within it.
   2. Enumerate each of those.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

1. List troubleshooting tips here.
2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

References

Relevant papers and books

1. Goldbeter A and Koshland DE Jr. An amplified sensitivity arising from covalent modification in biological systems. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6840-4. DOI:10.1073/pnas.78.11.6840 |
2. JACOB F and MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318-56. DOI:10.1016/s0022-2836(61)80072-7 |
3. ISBN:0879697164

Contact

• Who has experience with this protocol?

or instead, discuss this protocol.