NEB buffers

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; NEBuffer 4 (green) : 20 mM Tris-acetate, 10 mM magnesium acetate, 50 mM potassium acetate, 1 mM DTT (pH 7.9 at 25°C).
; NEBuffer 4 (green) : 20 mM Tris-acetate, 10 mM magnesium acetate, 50 mM potassium acetate, 1 mM DTT (pH 7.9 at 25°C).
-
; T4 DNA Ligase Buffer : 50 mM Tris-HCl, 10 mM MgCl2, 1 mM ATP, 10 mM Dithiothreitol, (pH 7.5 at 25°C).
+
; T4 DNA Ligase Buffer : 50 mM Tris-HCl, 10 mM MgCl2, 1 mM ATP, 10 mM Dithiothreitol (pH 7.5 at 25°C).
 +
 
 +
; NEBuffer EcoRI : 100 mM Tris-HCl, 50 mM NaCl, 10 mM MgCl2, 0.025 % Triton X-100 (pH 7.5 at 25°C).
Source: [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/buffer_legend.asp? NEB]
Source: [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/buffer_legend.asp? NEB]

Revision as of 18:47, 13 December 2007

Composition of NEBuffers (1x):

NEBuffer 1 (yellow) 
10 mM Bis Tris Propane-HCl, 10 mM MgCl2, 1 mM DTT (pH 7.0 at 25°C).
NEBuffer 2 (blue) 
10 mM Tris-HCl, 10 mM MgCl2, 50 mM NaCl, 1 mM DTT (pH 7.9 at 25°C).
NEBuffer 3 (red) 
50 mM Tris-HCl, 10 mM MgCl2, 100 mM NaCl, 1 mM DTT (pH 7.9 at 25°C).
NEBuffer 4 (green) 
20 mM Tris-acetate, 10 mM magnesium acetate, 50 mM potassium acetate, 1 mM DTT (pH 7.9 at 25°C).
T4 DNA Ligase Buffer 
50 mM Tris-HCl, 10 mM MgCl2, 1 mM ATP, 10 mM Dithiothreitol (pH 7.5 at 25°C).
NEBuffer EcoRI 
100 mM Tris-HCl, 50 mM NaCl, 10 mM MgCl2, 0.025 % Triton X-100 (pH 7.5 at 25°C).

Source: NEB

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