NEB buffers: Difference between revisions
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; NEBuffer 4 (green) : 20 mM Tris-acetate, 10 mM magnesium acetate, 50 mM potassium acetate, 1 mM DTT (pH 7.9 at 25°C). | ; NEBuffer 4 (green) : 20 mM Tris-acetate, 10 mM magnesium acetate, 50 mM potassium acetate, 1 mM DTT (pH 7.9 at 25°C). | ||
; T4 DNA Ligase Buffer : 50 mM Tris-HCl, 10 mM MgCl2, 1 mM ATP, 10 mM Dithiothreitol (pH 7.5 at 25°C). | |||
; NEBuffer EcoRI : 100 mM Tris-HCl, 50 mM NaCl, 10 mM MgCl2, 0.025 % Triton X-100 (pH 7.5 at 25°C). | |||
Source: [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/buffer_legend.asp? NEB] | Source: [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/buffer_legend.asp? NEB] | ||
[[Category:Material]] and [[Category:Buffers]] | |||
Latest revision as of 14:32, 11 March 2008
Composition of NEBuffers (1x):
- NEBuffer 1 (yellow)
- 10 mM Bis Tris Propane-HCl, 10 mM MgCl2, 1 mM DTT (pH 7.0 at 25°C).
- NEBuffer 2 (blue)
- 10 mM Tris-HCl, 10 mM MgCl2, 50 mM NaCl, 1 mM DTT (pH 7.9 at 25°C).
- NEBuffer 3 (red)
- 50 mM Tris-HCl, 10 mM MgCl2, 100 mM NaCl, 1 mM DTT (pH 7.9 at 25°C).
- NEBuffer 4 (green)
- 20 mM Tris-acetate, 10 mM magnesium acetate, 50 mM potassium acetate, 1 mM DTT (pH 7.9 at 25°C).
- T4 DNA Ligase Buffer
- 50 mM Tris-HCl, 10 mM MgCl2, 1 mM ATP, 10 mM Dithiothreitol (pH 7.5 at 25°C).
- NEBuffer EcoRI
- 100 mM Tris-HCl, 50 mM NaCl, 10 mM MgCl2, 0.025 % Triton X-100 (pH 7.5 at 25°C).
Source: NEB and
