NEB buffers

From OpenWetWare

Composition of NEBuffers (1x):

NEBuffer 1 (yellow) 

10 mM Bis Tris Propane-HCl, 10 mM MgCl2, 1 mM DTT (pH 7.0 at 25°C).

NEBuffer 2 (blue) 

10 mM Tris-HCl, 10 mM MgCl2, 50 mM NaCl, 1 mM DTT (pH 7.9 at 25°C).

NEBuffer 3 (red) 

50 mM Tris-HCl, 10 mM MgCl2, 100 mM NaCl, 1 mM DTT (pH 7.9 at 25°C).

NEBuffer 4 (green) 

20 mM Tris-acetate, 10 mM magnesium acetate, 50 mM potassium acetate, 1 mM DTT (pH 7.9 at 25°C).

Source: NEB