NRP-UEA-Norwich iGEM :Alumni pages

Return to: http://openwetware.org/wiki/NRP-UEA-Norwich

UEA iGEM Team 2011: UEA-JIC_Norwich

To see the team's iGEM wiki, go to://http://2011.igem.org/Team:UEA-JIC_Norwich

For this team, some Instructors and Advisors were based at the School of Biological Sciences at the University of East Anglia in Norwich (BIO) and some were based at the John Innes Centre in Norwich (JIC).

Instructors

• Richard Bowater (BIO)
• Matt Hutchings (BIO)
• Paul O'Maille (JIC)
• Richard Morris (JIC)
• Colwyn Thomas (BIO)
• Kay Yeoman (BIO)

Student Members

• Jarama Clucas
• Mario de Piano
• Gurdeep Dosanjh
• Ben Hardy
• Kimberley Hirst-Jones
• Ben Jevans
• Abigail Savoy
• Alistair Walsham
• Mark Wright

Advisors

• Steven Garrett (JIC)
• Richard Kelwick (BIO)

UEA iGEM Team 2012: NRP-UEA-Norwich

To see the team's iGEM wiki, go to://http://2012.igem.org/Team:NRP-UEA-Norwich

Project overview

Sensory BioBrick systems have been a large constituent of previous iGEM projects in which teams have combined impressive amounts of logic with limitless creativity in order to produce synthetically engineered organisms with the ability to detect the presence of specific substrates; this was achieved by combining various promoters and reporters to produce novel gene systems of great breadth and depth.

We too have taken a sensory approach to our project and have produced systems involved in the sensation of nitric oxide (NO). Originally we set out to develop a bacterial and mammalian hybrid NO-sensing promoter (which we have achieved); we then looked into ways of quantifying the levels of highly reactive and difficult to measure NO within a system, leading to us producing a novel gene regulation system known as the comparator circuit. Throughout the project we went on to look at theoretical alternative approaches to the gene systems we have produced.

For this team, all Instructors and Advisors were based at the School of Biological Sciences at the University of East Anglia in Norwich (BIO).

Instructors

• Richard Bowater (BIO)
• Matt Hutchings (BIO)
• Colwyn Thomas (BIO)
• Kay Yeoman (BIO)

Student Members

• Pascoe J Harvey
• Rachel Dobson
• Joy Edwards-Hicks
• Russell Gritton
• Lukas Harnisch
• Rebecca Lo
• Khadija Ouadi

Advisors

• Richard Kelwick (BIO)
• Amy Congdon (Mentor for art & film)

UEA iGEM Team 2013: NRP-UEA-Norwich

To see the team's iGEM wiki, go to://http://2013.igem.org/Team:NRP-UEA-Norwich

Project overview

Developing Biosensors to Identify Antimycin-Producing Actinomycetes

Antimycins, antifungal compounds primarily produced by Streptomyces (a sub-set of actinomycetes), function by inhibiting the final stage of the electron transport chain. Our aim was to develop Biosensors to aid identification of novel antimycin-producing actinomycetes.

Homologues of the AntA sigma factor, the key regulatory protein in antimycin biosynthesis, are present in all 14 known biosynthetic gene clusters. Due to this property, Biosensors have been designed with the AntA-regulated promoter (antGp) controlling the expression of three promoters: neomycin resistance gene, RFP (Red Fluorescent Protein) and GUS (providing beta-glucoronidase activity).

The Biosensors were produced, trialled and optimised where possible after sub-cloning into two actinomycete-specific integrative plasmids, pMS82 and pAU3-45. Worldwide soil and sediment samples were collected to produce a library of actinomycete strains, which were screened using our Biosensors, with the ultimate goal being to assess bacterial strains for actinomycin production.

For this team, all Instructors and Advisors were based at the School of Biological Sciences at the University of East Anglia in Norwich (BIO).

Instructors

• Richard Bowater (BIO)
• Matt Hutchings (BIO)
• Kay Yeoman (BIO)

Student Members

• Matt Batchelor
• Michael Brown
• Lucy Clark
• Shaima'a Hamarsheh
• Rebecca Spanner
• Divya Thankachan
• Beth Williams

Advisors

• John T. Munnoch (BIO)