NTA Chips for SPR: Difference between revisions
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*effects of pH, NaCl and detergent (tween) concentrations in the ligand immobilization buffer | *effects of pH, NaCl and detergent (tween) concentrations in the ligand immobilization buffer | ||
*effect of EDTA (0-300 mM, Na<sup>+</sup> salt) in the running and ligand buffers | *effect of EDTA (0-300 mM, Na<sup>+</sup> salt) in the running and ligand buffers | ||
<math>E=m \cdot c^2</math> | |||
Revision as of 19:22, 21 March 2008
Nieba et al. [1] investigated use of Ni2+-NTA chip surface for immobilization of his-tagged proteins.
Several parameters affecting level of ligand immobilization were studied:
- conditions for NTA surface activation with Ni2+: [NiCl2], [NaCl] concentrations and pH of activation buffer
- effects of pH, NaCl and detergent (tween) concentrations in the ligand immobilization buffer
- effect of EDTA (0-300 mM, Na+ salt) in the running and ligand buffers
[math]\displaystyle{ E=m \cdot c^2 }[/math]
