Nachury:Protocols: Difference between revisions
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==Protocols== | ==Protocols== | ||
=== | ===Extractology=== | ||
:*[[Nachury: | :*[[Nachury:Nuclear_Extract|Hypotonic/N<sub>2</sub> cavitation lysis]] | ||
:*[[Nachury: | :*[[Nachury:Testis_Extract|Testis extract]] | ||
:*[[Nachury: | :*[[Nachury:Retinal_Extract|Retinal extract]] | ||
:*[[Nachury: | :*[[Nachury:Retinal_Detergent_Extract|Retinal detergent extract]] | ||
===Protein Biochemistry=== | ===Protein Biochemistry=== | ||
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:*[[Nachury:MeOH_CHCl3|Methanol-Chloroform precipitation of proteins]] | :*[[Nachury:MeOH_CHCl3|Methanol-Chloroform precipitation of proteins]] | ||
:*[[Nachury:TCA|TCA precipitation of proteins]] | :*[[Nachury:TCA|TCA precipitation of proteins]] | ||
=== Immunofluorescence === | === Immunofluorescence === | ||
:*[[Nachury:General|General guidelines]] | :*[[Nachury:General|General guidelines]] | ||
:*[[Nachury:PFA_Saponin|PFA/Saponin]] | :*[[Nachury:PFA_Saponin|PFA/Saponin]] | ||
=== Lipids === | |||
:*[[Nachury:Liposome_Prep|Preparation of liposomes]] | |||
:*[[Nachury:Liposome_Binding|Protein-Liposomes interaction assays]] | |||
:*[[Nachury:PIP_strip|PIP strip blotting assays (fat blot)]] | |||
===DNA=== | ===DNA=== | ||
:*[[Nachury:CsCl_Maxiprep|Old-school maxiprep]] | :*[[Nachury:CsCl_Maxiprep|Old-school maxiprep]] | ||
Revision as of 19:50, 9 January 2008
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ProtocolsExtractology
Protein BiochemistryImmunofluorescenceLipidsDNA |
