Nachury:Protocols

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(Immunofluorescence)
(Protocols)
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==Protocols==
==Protocols==
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===Extracts===
+
===Extractology===
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:*[[Nachury:NuclearExtract|Hypotonic/N<sub>2</sub> cavitation lysis]]
+
:*[[Nachury:Nuclear_Extract|Hypotonic/N<sub>2</sub> cavitation lysis]]
-
:*[[Nachury:TestisExtract|Testis extract]]
+
:*[[Nachury:Testis_Extract|Testis extract]]
-
:*[[Nachury:TestisExtract|Retinal extract]]
+
:*[[Nachury:Retinal_Extract|Retinal extract]]
-
:*[[Nachury:TestisExtract|Retinal detergent extract]]
+
:*[[Nachury:Retinal_Detergent_Extract|Retinal detergent extract]]
 +
 
===Protein Biochemistry===
===Protein Biochemistry===
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:*[[Nachury:MeOH_CHCl3|Methanol-Chloroform precipitation of proteins]]
:*[[Nachury:MeOH_CHCl3|Methanol-Chloroform precipitation of proteins]]
:*[[Nachury:TCA|TCA precipitation of proteins]]
:*[[Nachury:TCA|TCA precipitation of proteins]]
-
 
=== Immunofluorescence ===
=== Immunofluorescence ===
:*[[Nachury:General|General guidelines]]
:*[[Nachury:General|General guidelines]]
:*[[Nachury:PFA_Saponin|PFA/Saponin]]
:*[[Nachury:PFA_Saponin|PFA/Saponin]]
 +
 +
=== Lipids ===
 +
:*[[Nachury:Liposome_Prep|Preparation of liposomes]]
 +
:*[[Nachury:Liposome_Binding|Protein-Liposomes interaction assays]]
 +
:*[[Nachury:PIP_strip|PIP strip blotting assays (fat blot)]]
===DNA===
===DNA===
:*[[Nachury:CsCl_Maxiprep|Old-school maxiprep]]
:*[[Nachury:CsCl_Maxiprep|Old-school maxiprep]]

Revision as of 21:50, 9 January 2008

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Protocols

Extractology


Protein Biochemistry

Immunofluorescence

Lipids

DNA

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