Current revision

Home Research People Publications Contact Resources

Protocols

@@ Line 2: / Line 2: @@
 __NOTOC__
 ==Protocols==
+=<center><font color="red">Under (very slow) construction</font></center>=
-===General===
+===Cell Lines===
-[[Nachury:silver|Silver staining]]
+:*[[Nachury:Retrovirus_Infection|Establishing stable lines with retrovirus]]
-[[Nachury:MeOH_CHCl3|Methanol-Chloroform precipitation of proteins]]
+:*[[Nachury:FACS_Cloning|Cloning fluorescent cells by FACS]]
-[[Nachury:TCA|TCA precipitation of proteins]]
+:*[[Nachury:Cell_expansion|Growing and harvesting cells in large amounts]]
+===Extractology===
+:*[[Nachury:Nuclear_Extract|Hypotonic/N<sub>2</sub> cavitation lysis]]
+:*[[Nachury:Testis_Extract|Testis extract]]
+:*[[Nachury:Retinal_Extract|Retinal extract]]
+:*[[Nachury:Retinal_Detergent_Extract|Retinal detergent extract]]
+===Protein Biochemistry===
+:*[[Nachury:TAP|TAP purification of protein complexes from mammalian cells]]
+:*[[Nachury:silver|Silver staining]]
+:*[[Nachury:MeOH_CHCl3|Methanol-Chloroform precipitation of proteins]]
+:*[[Nachury:TCA|TCA precipitation of proteins]]
+=== Immunofluorescence ===
+:*[[Nachury:General|General guidelines]]
+:*[[Nachury:PFA_Saponin|PFA/Saponin]]
+=== Lipids ===
+:*[[Nachury:Liposome_Prep|Preparation of liposomes]]
+:*[[Nachury:Liposome_Binding|Protein-Liposomes interaction assays]]
+:*[[Nachury:PIP_strip|PIP strip blotting assays (fat blot)]]
+===DNA===
+:*[[Nachury:CsCl_Maxiprep|Old-school maxiprep]]
+=== Microtubules ===