Nachury:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(8 intermediate revisions by the same user not shown) | |||
Line 2: | Line 2: | ||
__NOTOC__ | __NOTOC__ | ||
==Protocols== | ==Protocols== | ||
=<center><font color="red">Under (very slow) construction</font></center>= | |||
=== | ===Cell Lines=== | ||
:*[[Nachury: | :*[[Nachury:Retrovirus_Infection|Establishing stable lines with retrovirus]] | ||
:*[[Nachury: | :*[[Nachury:FACS_Cloning|Cloning fluorescent cells by FACS]] | ||
:*[[Nachury: | :*[[Nachury:Cell_expansion|Growing and harvesting cells in large amounts]] | ||
:*[[Nachury: | |||
===Extractology=== | |||
:*[[Nachury:Nuclear_Extract|Hypotonic/N<sub>2</sub> cavitation lysis]] | |||
:*[[Nachury:Testis_Extract|Testis extract]] | |||
:*[[Nachury:Retinal_Extract|Retinal extract]] | |||
:*[[Nachury:Retinal_Detergent_Extract|Retinal detergent extract]] | |||
===Protein Biochemistry=== | ===Protein Biochemistry=== | ||
:*[[Nachury:TAP|TAP purification of protein complexes from mammalian cells]] | |||
:*[[Nachury:silver|Silver staining]] | :*[[Nachury:silver|Silver staining]] | ||
:*[[Nachury:MeOH_CHCl3|Methanol-Chloroform precipitation of proteins]] | :*[[Nachury:MeOH_CHCl3|Methanol-Chloroform precipitation of proteins]] | ||
:*[[Nachury:TCA|TCA precipitation of proteins]] | :*[[Nachury:TCA|TCA precipitation of proteins]] | ||
=== Immunofluorescence === | |||
:*[[Nachury:General|General guidelines]] | |||
:*[[Nachury:PFA_Saponin|PFA/Saponin]] | |||
=== Lipids === | |||
:*[[Nachury:Liposome_Prep|Preparation of liposomes]] | |||
:*[[Nachury:Liposome_Binding|Protein-Liposomes interaction assays]] | |||
:*[[Nachury:PIP_strip|PIP strip blotting assays (fat blot)]] | |||
===DNA=== | ===DNA=== | ||
:*[[Nachury:CsCl_Maxiprep|Old-school maxiprep]] | :*[[Nachury:CsCl_Maxiprep|Old-school maxiprep]] | ||
=== Microtubules === |
Latest revision as of 12:37, 2 November 2011
<html> <img src="http://openwetware.org/images/e/ea/Nachury_banner2.jpg" width="750"> </html>
Protocols
|