Nachury:Protocols
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==Protocols== | ==Protocols== | ||
| + | =<center><font color="red">Under (very slow) construction</font></center>= | ||
| - | === | + | ===Cell Lines=== |
| - | :*[[Nachury: | + | :*[[Nachury:Retrovirus_Infection|Establishing stable lines with retrovirus]] |
| - | :*[[Nachury: | + | :*[[Nachury:FACS_Cloning|Cloning fluorescent cells by FACS]] |
| - | :*[[Nachury: | + | :*[[Nachury:Cell_expansion|Growing and harvesting cells in large amounts]] |
| - | :*[[Nachury: | + | |
| + | ===Extractology=== | ||
| + | :*[[Nachury:Nuclear_Extract|Hypotonic/N<sub>2</sub> cavitation lysis]] | ||
| + | :*[[Nachury:Testis_Extract|Testis extract]] | ||
| + | :*[[Nachury:Retinal_Extract|Retinal extract]] | ||
| + | :*[[Nachury:Retinal_Detergent_Extract|Retinal detergent extract]] | ||
===Protein Biochemistry=== | ===Protein Biochemistry=== | ||
| + | :*[[Nachury:TAP|TAP purification of protein complexes from mammalian cells]] | ||
:*[[Nachury:silver|Silver staining]] | :*[[Nachury:silver|Silver staining]] | ||
:*[[Nachury:MeOH_CHCl3|Methanol-Chloroform precipitation of proteins]] | :*[[Nachury:MeOH_CHCl3|Methanol-Chloroform precipitation of proteins]] | ||
:*[[Nachury:TCA|TCA precipitation of proteins]] | :*[[Nachury:TCA|TCA precipitation of proteins]] | ||
| + | |||
| + | === Immunofluorescence === | ||
| + | :*[[Nachury:General|General guidelines]] | ||
| + | :*[[Nachury:PFA_Saponin|PFA/Saponin]] | ||
| + | |||
| + | === Lipids === | ||
| + | :*[[Nachury:Liposome_Prep|Preparation of liposomes]] | ||
| + | :*[[Nachury:Liposome_Binding|Protein-Liposomes interaction assays]] | ||
| + | :*[[Nachury:PIP_strip|PIP strip blotting assays (fat blot)]] | ||
===DNA=== | ===DNA=== | ||
:*[[Nachury:CsCl_Maxiprep|Old-school maxiprep]] | :*[[Nachury:CsCl_Maxiprep|Old-school maxiprep]] | ||
| + | |||
| + | === Microtubules === | ||
Current revision
Protocols
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