Nachury:Protocols

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(Protocols)
Current revision (14:37, 2 November 2011) (view source)
(Protocols)
 
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==Protocols==
==Protocols==
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=<center><font color="red">Under (very slow) construction</font></center>=
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===Extracts===
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===Cell Lines===
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:*[[Nachury:NuclearExtract|Hypotonic/N<sub>2</sub> cavitation lysis]]
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:*[[Nachury:Retrovirus_Infection|Establishing stable lines with retrovirus]]
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:*[[Nachury:TestisExtract|Testis extract]]
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:*[[Nachury:FACS_Cloning|Cloning fluorescent cells by FACS]]
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:*[[Nachury:TestisExtract|Retinal extract]]
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:*[[Nachury:Cell_expansion|Growing and harvesting cells in large amounts]]
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:*[[Nachury:TestisExtract|Retinal detergent extract]]
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===Extractology===
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:*[[Nachury:Nuclear_Extract|Hypotonic/N<sub>2</sub> cavitation lysis]]
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:*[[Nachury:Testis_Extract|Testis extract]]
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:*[[Nachury:Retinal_Extract|Retinal extract]]
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:*[[Nachury:Retinal_Detergent_Extract|Retinal detergent extract]]
===Protein Biochemistry===
===Protein Biochemistry===
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:*[[Nachury:TAP|TAP purification of protein complexes from mammalian cells]]
:*[[Nachury:silver|Silver staining]]
:*[[Nachury:silver|Silver staining]]
:*[[Nachury:MeOH_CHCl3|Methanol-Chloroform precipitation of proteins]]
:*[[Nachury:MeOH_CHCl3|Methanol-Chloroform precipitation of proteins]]
:*[[Nachury:TCA|TCA precipitation of proteins]]
:*[[Nachury:TCA|TCA precipitation of proteins]]
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=== Immunofluorescence ===
=== Immunofluorescence ===
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:*[[Nachury:General|General guidelines]]
:*[[Nachury:PFA_Saponin|PFA/Saponin]]
:*[[Nachury:PFA_Saponin|PFA/Saponin]]
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=== Lipids ===
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:*[[Nachury:Liposome_Prep|Preparation of liposomes]]
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:*[[Nachury:Liposome_Binding|Protein-Liposomes interaction assays]]
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:*[[Nachury:PIP_strip|PIP strip blotting assays (fat blot)]]
===DNA===
===DNA===
:*[[Nachury:CsCl_Maxiprep|Old-school maxiprep]]
:*[[Nachury:CsCl_Maxiprep|Old-school maxiprep]]
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=== Microtubules ===

Current revision

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Protocols

Under (very slow) construction

Cell Lines

Extractology

Protein Biochemistry

Immunofluorescence

Lipids

DNA

Microtubules

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