Nachury:Protocols: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
 
(4 intermediate revisions by the same user not shown)
Line 2: Line 2:
__NOTOC__
__NOTOC__
==Protocols==
==Protocols==
=<center><font color="red">Under (very slow) construction</font></center>=
===Cell Lines===
:*[[Nachury:Retrovirus_Infection|Establishing stable lines with retrovirus]]
:*[[Nachury:FACS_Cloning|Cloning fluorescent cells by FACS]]
:*[[Nachury:Cell_expansion|Growing and harvesting cells in large amounts]]


===Extractology===
===Extractology===
Line 8: Line 14:
:*[[Nachury:Retinal_Extract|Retinal extract]]
:*[[Nachury:Retinal_Extract|Retinal extract]]
:*[[Nachury:Retinal_Detergent_Extract|Retinal detergent extract]]
:*[[Nachury:Retinal_Detergent_Extract|Retinal detergent extract]]


===Protein Biochemistry===
===Protein Biochemistry===
:*[[Nachury:TAP|TAP purification of protein complexes from mammalian cells]]
:*[[Nachury:silver|Silver staining]]
:*[[Nachury:silver|Silver staining]]
:*[[Nachury:MeOH_CHCl3|Methanol-Chloroform precipitation of proteins]]
:*[[Nachury:MeOH_CHCl3|Methanol-Chloroform precipitation of proteins]]
Line 26: Line 32:
===DNA===
===DNA===
:*[[Nachury:CsCl_Maxiprep|Old-school maxiprep]]
:*[[Nachury:CsCl_Maxiprep|Old-school maxiprep]]
=== Microtubules ===

Latest revision as of 12:37, 2 November 2011