Nachury:Protocols

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(Protocols)
Current revision (14:37, 2 November 2011) (view source)
(Protocols)
 
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__NOTOC__
__NOTOC__
==Protocols==
==Protocols==
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<center><font color="red">Under construction</font></center>
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=<center><font color="red">Under (very slow) construction</font></center>=
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===Cell Lines===
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:*[[Nachury:Retrovirus_Infection|Establishing stable lines with retrovirus]]
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:*[[Nachury:FACS_Cloning|Cloning fluorescent cells by FACS]]
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:*[[Nachury:Cell_expansion|Growing and harvesting cells in large amounts]]
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===Extractology===
===Extractology===
:*[[Nachury:Nuclear_Extract|Hypotonic/N<sub>2</sub> cavitation lysis]]
:*[[Nachury:Nuclear_Extract|Hypotonic/N<sub>2</sub> cavitation lysis]]
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:*[[Nachury:Retinal_Extract|Retinal extract]]
:*[[Nachury:Retinal_Extract|Retinal extract]]
:*[[Nachury:Retinal_Detergent_Extract|Retinal detergent extract]]
:*[[Nachury:Retinal_Detergent_Extract|Retinal detergent extract]]
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===Protein Biochemistry===
===Protein Biochemistry===
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:*[[Nachury:TAP|TAP purification of protein complexes from mammalian cells]]
:*[[Nachury:silver|Silver staining]]
:*[[Nachury:silver|Silver staining]]
:*[[Nachury:MeOH_CHCl3|Methanol-Chloroform precipitation of proteins]]
:*[[Nachury:MeOH_CHCl3|Methanol-Chloroform precipitation of proteins]]
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===DNA===
===DNA===
:*[[Nachury:CsCl_Maxiprep|Old-school maxiprep]]
:*[[Nachury:CsCl_Maxiprep|Old-school maxiprep]]
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=== Microtubules ===

Current revision

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Protocols

Under (very slow) construction

Cell Lines

Extractology

Protein Biochemistry

Immunofluorescence

Lipids

DNA

Microtubules

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