Nachury:Protocols

Protocols

Cell Lines

• Establishing stable lines with retrovirus
• Cloning fluorescent cells by FACS
• Growing and harvesting cells in large amounts

Extractology

• Hypotonic/N₂ cavitation lysis
• Testis extract
• Retinal extract
• Retinal detergent extract

Protein Biochemistry

• TAP purification of protein complexes from mammalian cells
• Silver staining
• Methanol-Chloroform precipitation of proteins
• TCA precipitation of proteins

Immunofluorescence

• General guidelines
• PFA/Saponin

Lipids

• Preparation of liposomes
• Protein-Liposomes interaction assays
• PIP strip blotting assays (fat blot)

DNA

• Old-school maxiprep