NanoBio:Protocols: Difference between revisions
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=Nano Synth Bio= | =Nano Synth Bio= | ||
==General Molecular Biology== | ==General Molecular Biology== | ||
[[Current Protocols in Molecular Biology]] | [[http://www.mrw.interscience.wiley.com/cp/cpmb/cpmb_contents_fs.html|Current Protocols in Molecular Biology]] | ||
==Sub-cloning using Biobricks or Biofusion Vectors== | ==Sub-cloning using Biobricks or Biofusion Vectors== | ||
*Note: Many of these are protocols that I used and/or developed as a postdoctoral fellow in Pam Silver's lab. | *Note: Many of these are protocols that I used and/or developed as a postdoctoral fellow in Pam Silver's lab. | ||
Revision as of 11:05, 4 December 2007
Nano Synth Bio
General Molecular Biology
[Protocols in Molecular Biology]
Sub-cloning using Biobricks or Biofusion Vectors
- Note: Many of these are protocols that I used and/or developed as a postdoctoral fellow in Pam Silver's lab.
- Strategy for Making Parts
- Designing Parts or Genes
- Commonly Used Plasmids
- Commonly Used Primers
- PCR
- Oligonucleotide Inserts
- Overlapping Oligonucleotide Inserts
- Site-Directed Mutagenesis
- Restriction Digest
- Ligation
- Quick & Dirty Digest & Ligation
- Bacterial Transformation
- Plasmid Verification
Genomic Integration into S. cerevisiae
Lipid Bilayers
Care of Synechocystis sp 6803
Primers
- Biobricks & Biofusion Sub-cloning
- Synechocystis sp. 6803
- Guidelines on Talking or Writing about your project
- Microbial Respiration
