NanoBio:Protocols
From OpenWetWare
(Difference between revisions)
(→Sub-cloning using Biobricks or Biofusion Vectors) |
(→Sub-cloning using Biobricks or Biofusion Vectors) |
||
| Line 11: | Line 11: | ||
*[[NanoBio: PCR | PCR]] | *[[NanoBio: PCR | PCR]] | ||
*[[NanoBio: Oligonucleotide Inserts | Oligonucleotide Inserts]] | *[[NanoBio: Oligonucleotide Inserts | Oligonucleotide Inserts]] | ||
| - | *[[ | + | *[[NanoBio: Overlapping Oligonucleotide Inserts | Overlapping Oligonucleotide Inserts]] |
*[[Silver: Site-Directed Mutagenesis | Site-Directed Mutagenesis]] | *[[Silver: Site-Directed Mutagenesis | Site-Directed Mutagenesis]] | ||
*[[Silver: Restriction Digest | Restriction Digest]] | *[[Silver: Restriction Digest | Restriction Digest]] | ||
Revision as of 14:35, 26 January 2008
Contents |
Nano Synth Bio
General Molecular Biology
Current Protocols in Molecular Biology
Sub-cloning using Biobricks or Biofusion Vectors
- Note: Many of these are protocols that I used and/or developed as a postdoctoral fellow in Pam Silver's lab.
- Strategy for Making Parts
- Designing Parts or Genes
- Commonly Used Plasmids
- Commonly Used Primers
- PCR
- Oligonucleotide Inserts
- Overlapping Oligonucleotide Inserts
- Site-Directed Mutagenesis
- Restriction Digest
- Ligation
- Quick & Dirty Digest & Ligation
- Bacterial Transformation
- Plasmid Verification
Genomic Integration into S. cerevisiae
Lipid Bilayers
Care of Synechocystis sp 6803
Primers
- Biobricks & Biofusion Sub-cloning
- Synechocystis sp. 6803
- Shewanella oneidensis MR-1
- Guidelines on Talking or Writing about your project
- Microbial Respiration
